Biochemical characterisation of methanethiol oxidase: a key enzyme of volatile organosulfur compound degradation

甲硫醇氧化酶的生化表征:挥发性有机硫化合物降解的关键酶

基本信息

  • 批准号:
    BB/H003851/1
  • 负责人:
  • 金额:
    $ 44.52万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2009
  • 资助国家:
    英国
  • 起止时间:
    2009 至 无数据
  • 项目状态:
    已结题

项目摘要

We propose to characterise the properties of a bacterial enzyme that converts a foul smelling, toxic gaseous organic sulfur compound. Methanethiol oxidase converts methanethiol - a gas smelling of rotten cabbage - which is an important sulfur compound in nature. The products of the methanethiol catalysed reaction are formaldehyde, hydrogen sulfide (smells of rotten eggs) and hydrogen peroxide (a chemical that is used as a bleaching agent in contact lens disinfection fluids). Very little is known of the molecular and catalytic properties of methanethiol oxidases. The work we propose will provide fundamental insights into the biochemistry of methanethiol degradation, a process that on a global scale is responsible for turnover of some 300-500 million tons of sulfur in the oceans alone, where methanethiol occurs as a degradation product of a climate cooling gas (dimethylsulfide) and its precursor molecule (dimethylsulfoniopropionate). Methanethiol plays a role as a flavour compound in foods and beverages, but can also contribute to what we perceive as off-flavours when it occurs at too high concentrations. In an industrial context, methanethiol is also significant as a degradation product of bulk solvents (dimethylsulfoxide) and it is conceivable that methanethiol oxidase could find application as an industrial biocatalyst. Our preliminary data also indicate that this enzyme may work in concert with other enzymes in a complex of proteins inside the cell, which may protect it to some degree from its toxic intermediates and may enhance its function. This enzyme complex will also be studied further. An interesting aspect of methanethiol oxidase that we have just discovered is that this bacterial enzyme is encoded by a gene for which there has not been any information indicating its function previously. It is hence of interest to further investigate the enzyme, in particular as there are similar enzymes encoded by genomes of animals including humans on whose function similarly little is known. Thus, studying this bacterial enzyme in more detail, may shed light on the evolution of a type of protein which is also found in our bodies. The ultimate goal of characterising an enzyme is to elucidate its three dimensional structure. While this is not planned in this project, we aim to make the first step towards achieving that goal by producing crystals of the enzyme that can be characterised by X-ray diffraction analysis. This will require using recombinant DNA technology, which will allow to produce sufficient amounts of the enzyme for crystallisation.
我们提议描述一种细菌酶的特性,这种酶可以转化一种恶臭的、有毒的气态有机硫化合物。甲烷硫醇氧化酶能转化甲烷硫醇——一种有腐烂卷心菜气味的气体——这是自然界中一种重要的含硫化合物。甲硫醇催化反应的产物是甲醛、硫化氢(臭鸡蛋的气味)和过氧化氢(一种在隐形眼镜消毒液中用作漂白剂的化学物质)。对甲硫醇氧化酶的分子和催化性质所知甚少。我们提出的工作将为甲硫醇降解的生物化学提供基本的见解,这一过程在全球范围内仅在海洋中就负责约3 -5亿吨硫的周转量,其中甲硫醇作为气候冷却气体(二甲基硫化物)及其前体分子(二甲基磺酰丙酸酯)的降解产物发生。甲烷硫醇在食品和饮料中扮演着调味化合物的角色,但当它的浓度过高时,也会导致我们认为的异味。在工业环境中,甲硫醇作为散装溶剂(二甲基亚砜)的降解产物也很重要,可以想象,甲硫醇氧化酶可以作为工业生物催化剂得到应用。我们的初步数据还表明,这种酶可能与细胞内蛋白质复合体中的其他酶协同工作,这可能在一定程度上保护它免受有毒中间体的侵害,并可能增强其功能。这种酶复合物也将进一步研究。我们刚刚发现的甲硫醇氧化酶的一个有趣的方面是,这种细菌酶是由一个基因编码的,而之前没有任何信息表明它的功能。因此,进一步研究这种酶是很有意义的,特别是在包括人类在内的动物基因组编码的类似酶的情况下,对其功能知之甚少。因此,更详细地研究这种细菌酶,可能会揭示一种蛋白质的进化,这种蛋白质也存在于我们的体内。表征酶的最终目的是阐明它的三维结构。虽然这不是本项目的计划,但我们的目标是通过生产可以通过x射线衍射分析表征的酶晶体,迈出实现这一目标的第一步。这将需要使用重组DNA技术,这将允许生产足够数量的酶用于结晶。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Bacterial SBP56 identified as a Cu-dependent methanethiol oxidase widely distributed in the biosphere.
  • DOI:
    10.1038/ismej.2017.148
  • 发表时间:
    2018-01
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Eyice Ö;Myronova N;Pol A;Carrión O;Todd JD;Smith TJ;Gurman SJ;Cuthbertson A;Mazard S;Mennink-Kersten MA;Bugg TD;Andersson KK;Johnston AW;Op den Camp HJ;Schäfer H
  • 通讯作者:
    Schäfer H
Mutations in SELENBP1, encoding a novel human methanethiol oxidase, cause extraoral halitosis.
  • DOI:
    10.1038/s41588-017-0006-7
  • 发表时间:
    2018-01
  • 期刊:
  • 影响因子:
    30.8
  • 作者:
    Pol A;Renkema GH;Tangerman A;Winkel EG;Engelke UF;de Brouwer APM;Lloyd KC;Araiza RS;van den Heuvel L;Omran H;Olbrich H;Oude Elberink M;Gilissen C;Rodenburg RJ;Sass JO;Schwab KO;Schäfer H;Venselaar H;Sequeira JS;Op den Camp HJM;Wevers RA
  • 通讯作者:
    Wevers RA
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Hendrik Schaefer其他文献

Hendrik Schaefer的其他文献

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{{ truncateString('Hendrik Schaefer', 18)}}的其他基金

The tree phyllosphere microbiome - an overlooked and important sink for carbon monoxide?
树木叶际微生物群——一个被忽视的重要一氧化碳库?
  • 批准号:
    NE/X001245/1
  • 财政年份:
    2023
  • 资助金额:
    $ 44.52万
  • 项目类别:
    Research Grant
SIMbRICS: Sea Ice Microbiology and the Role In Cycling of Sulfur (DMS, DMSP, DMSO, MT)
SIMbRICS:海冰微生物学和硫循环中的作用(DMS、DMSP、DMSO、MT)
  • 批准号:
    NE/S002596/1
  • 财政年份:
    2019
  • 资助金额:
    $ 44.52万
  • 项目类别:
    Research Grant
Is bacterial DMS consumption dependent on methylamines in marine waters?
细菌 DMS 消耗量是否取决于海水中的甲胺?
  • 批准号:
    NE/R010404/1
  • 财政年份:
    2018
  • 资助金额:
    $ 44.52万
  • 项目类别:
    Research Grant
Microbial degradation of dimethylsulfoxide in the marine environment
海洋环境中二甲亚砜的微生物降解
  • 批准号:
    NE/L006448/1
  • 财政年份:
    2014
  • 资助金额:
    $ 44.52万
  • 项目类别:
    Research Grant
Stable Isotope Probing-metagenomics of river microbial populations degrading the aromatic pollutant para-nitrophenol (PNP)
降解芳香族污染物对硝基苯酚 (PNP) 的河流微生物种群的稳定同位素探测宏基因组学
  • 批准号:
    NE/J014168/1
  • 财政年份:
    2012
  • 资助金额:
    $ 44.52万
  • 项目类别:
    Research Grant
Making and breaking DMS by salt marsh microbes - populations and pathways, revealed by stable isotope probing and molecular techniques
盐沼微生物制造和破坏 DMS - 通过稳定同位素探测和分子技术揭示的种群和途径
  • 批准号:
    NE/H008918/1
  • 财政年份:
    2010
  • 资助金额:
    $ 44.52万
  • 项目类别:
    Research Grant
Genome sequencing of lytic and temperate phages infecting members of the Roseobacter clade
感染玫瑰杆菌分支成员的裂解噬菌体和温带噬菌体的基因组测序
  • 批准号:
    NE/F010044/1
  • 财政年份:
    2008
  • 资助金额:
    $ 44.52万
  • 项目类别:
    Research Grant
Marine microbial degradation of dimethylsulfide: Process understanding through application of postgenomic approaches to a model organism
二甲基硫醚的海洋微生物降解:通过对模型生物应用后基因组方法来理解过程
  • 批准号:
    NE/E013333/1
  • 财政年份:
    2007
  • 资助金额:
    $ 44.52万
  • 项目类别:
    Fellowship

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确认和描述宜居外星世界的途径
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