FUNCTIONAL ANTIGENS OF TRICHINELLA SPIRALIS

拟议研究的目标是纯化和表征所有 旋毛虫感染性L1幼虫分泌的抗原 螺旋形的 纯化将使用富含抗原的 通过免疫亲和色谱法分离感染性L1幼虫的组分， 克隆抗体 将测定每种纯化抗原诱导保护的能力 对抗小鼠的口腔攻击感染。 保护诱导抗原 将在物理（分子量）、化学（总 碳水化合物含量，氨基糖，氨基酸组成，胰蛋白酶消化 指纹）和生物学（在感染性L1幼虫内的定位， 在正常人中，在感染的肠内阶段期间宿主组织中的命运 和免疫宿主）。 所有纯化抗原将用于微量ELISA 试验，以评估其在猪血清学诊断中的有用性， 人旋毛虫病

The goal of the proposed research is to purify and characterize all antigens which are secreted by the infective L1 larva of Trichinella spiralis. Purifications will be accomplished employing antigen-enriched fractions of infective L1 larvae by immuno-affinity chromatography and monoclonal antibodies. Each purified antigen will be assayed for its ability to induce protection against an oral challenge infection in mice. Protection-inducing antigens will be characterized physically (molecular weight), chemically (total carbohydrate content, amino sugars, amino acid composition, tryptic digest fingerprint), and biologically (localization within the infective L1 larva, fate in host tissues during the enteral phase of infection in the normal and immunized host). All purified antigens will be used in the micro-ELISA test in order to assess their usefulness in the serodiagnosis of swine and human trichinellosis.