FUNCTIONAL ANTIGENS OF TRICHINELLA SPIRALIS

拟议研究的目标是提纯和描述所有 旋毛虫感染L1幼虫分泌的抗原 螺旋藻。纯化将使用浓缩的抗原来完成 免疫亲和层析分离感染L1幼虫的组分 单克隆抗体。 每个纯化的抗原都将被检测其诱导保护的能力。 在小鼠中对抗口服挑战感染。保护性诱导抗原 将表征物理(分子量)、化学(总 碳水化合物含量、氨基糖、氨基酸组成、胰酶消化 指纹)和生物学(定位于感染的L1幼虫内， 正常人肠道感染过程中宿主组织的去向 和免疫宿主)。所有纯化的抗原都将用于微量酶联免疫吸附试验 以评估它们在猪和猪的血清诊断中的有效性 人类旋毛虫病。

The goal of the proposed research is to purify and characterize all antigens which are secreted by the infective L1 larva of Trichinella spiralis. Purifications will be accomplished employing antigen-enriched fractions of infective L1 larvae by immuno-affinity chromatography and monoclonal antibodies. Each purified antigen will be assayed for its ability to induce protection against an oral challenge infection in mice. Protection-inducing antigens will be characterized physically (molecular weight), chemically (total carbohydrate content, amino sugars, amino acid composition, tryptic digest fingerprint), and biologically (localization within the infective L1 larva, fate in host tissues during the enteral phase of infection in the normal and immunized host). All purified antigens will be used in the micro-ELISA test in order to assess their usefulness in the serodiagnosis of swine and human trichinellosis.