ANTIGEN RECOGNITITON BY CYTOTOXIC T LYMPHOCYTES

这项工作涉及阐明被识别的抗原决定因素 细胞溶解胸腺依赖淋巴细胞(CTL)。我们已经发现了这种多肽 仙台病毒和流感病毒的糖蛋白片段都可以刺激这些 小鼠脾细胞体外CTL。一个主要目标是隔离和 对刺激片段的结构进行生化表征。我们会 进一步测试这些片段在体内的生理作用 抗体的诱导和CTL的形成。此外，我们还将表演 与Thomas Braciale博士在圣路易斯合作进行实验 我们的片段与克隆T细胞在长期培养中的相互作用 了解这些片段与单个T细胞的直接相互作用。我们 还将尝试将流感糖蛋白从脂质体转移到 以仙台HN和F蛋白为载体的CTL靶细胞。

This work concerns the elucidation of the antigenic determinants recognized by cytolytic thymus-dependent lymphocytes (CTL). We have found that peptide fragments of both Sendai and influenza viral glycoproteins can stimulate these CTL in vitro from mouse spleen cells. One major goal is to isolate and characterize biochemically the structure of the stimulatory fragments. We will further test the physiological roles of these fragments in vivo with respect to induction of antibody and CTL formation. We will, in addition, be performing collaborative experiments together with Dr. Thomas Braciale in St. Louis to test the interactions of our fragments with cloned T cells in long term culture to understand the direct interaction of the fragments with individual T cells. We will also attempt to transfer the influenza glycoproteins from a liposome to a target cell for CTL using the Sendai HN and F proteins as carriers.