ANTIGEN RECOGNITITON BY CYTOTOXIC T LYMPHOCYTES

这项工作涉及阐明的抗原决定簇识别的 溶细胞胸腺依赖性淋巴细胞（CTL）。 我们发现这种肽 仙台病毒和流感病毒糖蛋白的片段可以刺激这些 来自小鼠脾细胞的体外CTL。 一个主要目标是隔离和 生物化学地表征刺激片段的结构。 我们将 进一步测试这些片段在体内的生理作用， 诱导抗体和CTL形成。 此外，我们还将表演 与圣路易斯的托马斯·布拉西亚莱博士合作进行实验， 我们的片段与克隆T细胞在长期培养中的相互作用， 了解片段与单个T细胞的直接相互作用。 我们 也将尝试将流感糖蛋白从脂质体转移到 使用仙台HN和F蛋白作为载体的CTL的靶细胞。

This work concerns the elucidation of the antigenic determinants recognized by cytolytic thymus-dependent lymphocytes (CTL). We have found that peptide fragments of both Sendai and influenza viral glycoproteins can stimulate these CTL in vitro from mouse spleen cells. One major goal is to isolate and characterize biochemically the structure of the stimulatory fragments. We will further test the physiological roles of these fragments in vivo with respect to induction of antibody and CTL formation. We will, in addition, be performing collaborative experiments together with Dr. Thomas Braciale in St. Louis to test the interactions of our fragments with cloned T cells in long term culture to understand the direct interaction of the fragments with individual T cells. We will also attempt to transfer the influenza glycoproteins from a liposome to a target cell for CTL using the Sendai HN and F proteins as carriers.