ANTIGEN RECOGNITITON BY CYTOTOXIC T LYMPHOCYTES

细胞毒性 T 淋巴细胞对抗原的识别

• 批准号: 3126005
• 负责人: DAVID P FAN
• 金额: $ 14.76万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-08-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3126005
• 关键词: Staphylococcus aureus; T lymphocyte; antigens; beta globulin; cell fusion; cellular immunity; human tissue; leukocyte activation /transformation; macrophage; membrane reconstitution /synthesis; parainfluenza virus type 1; radiotracer; serology /serodiagnosis; spleen; tissue /cell culture; virus antigen

This work concerns the elucidation of the antigenic determinants recognized by cytolytic thymus-dependent lymphocytes (CTL). We have found that peptide fragments of both Sendai and influenza viral glycoproteins can stimulate these CTL in vitro from mouse spleen cells. One major goal is to isolate and characterize biochemically the structure of the stimulatory fragments. We will further test the physiological roles of these fragments in vivo with respect to induction of antibody and CTL formation. We will, in addition, be performing collaborative experiments together with Dr. Thomas Braciale in St. Louis to test the interactions of our fragments with cloned T cells in long term culture to understand the direct interaction of the fragments with individual T cells. We will also attempt to transfer the influenza glycoproteins from a liposome to a target cell for CTL using the Sendai HN and F proteins as carriers.

这项工作涉及到的抗原决定因子的阐明