CYTOLYTIC T LYMPHOCYTE HELPER FACTOR

溶细胞T淋巴细胞辅助因子

• 批准号: 3172815
• 负责人: DAVID P FAN
• 金额: $ 8.78万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3172815
• 关键词: T lymphocyte; cytokine; high performance liquid chromatography; immunochemistry; interferons; interleukin 2; leukocyte activation /transformation; major histocompatibility complex; monoclonal antibody; tissue /cell culture; tumor antigens

We recently found that the in vitro generation of cytolytic T lymphocytes (CTL) from mouse spleen cells requires added soluble factors under some circumstances. These include the: (1) generation of CTL specific for alloantigens when the antigen is on tumor cells and the responding spleen cells are from nonimmunized mice; (2) generation of CTL specific for syngeneic tumor antigens when the responding spleen cells are from nonimmunized mice; and (3) generation of antiviral CTL from precursors from immunized mice if certain viral peptides are used. We have begun to characterize the needed factors biochemically and have found that the factor needed for the allogeneic response can come from phorbol myristic acetate and Concanavalin A-stimulated ROM4 cells and from nonstimulated WEHI-3, AFSTL1 and AFSTL2 cells. The WEHI-3 factor is interleukin-3 which also functions in assisting the generation of antiviral CTL using peptide antigens. Our major objective is to purify and characterize the new factors from ROM4, AFSTL1, and AFSTL2 cells both biochemically and functionally. (HF)

我们最近发现体外产生的溶细胞性T淋巴细胞 (CTL)从小鼠脾细胞需要添加可溶性因子， 情节 这些包括：（1）产生特异于 当抗原位于肿瘤细胞和应答脾上时， 细胞来自未免疫的小鼠;（2）产生特异于 同源肿瘤抗原，当应答脾细胞来自 非免疫小鼠;和（3）从来自小鼠的前体产生抗病毒CTL。 如果使用某些病毒肽，则免疫小鼠。 我们已经开始从生物化学的角度来描述所需的因素， 发现同种异体反应所需的因子可以来自于 佛波醇肉豆蔻乙酸酯和伴刀豆球蛋白A刺激的ROM 4细胞和来自 未刺激的WEHI-3、AFSTL 1和AFSTL 2细胞。 WEHI-3因子为 白细胞介素-3也可以帮助产生抗病毒 使用肽抗原的CTL。 我们的主要目标是纯化和表征新的因素， ROM 4、AFSTL 1和AFSTL 2细胞的生物化学和功能。 （HF）