CYTOLYTIC T LYMPHOCYTE HELPER FACTOR

我们最近发现，体外产生的细胞溶解T淋巴细胞 小鼠脾细胞(CTL)在某些条件下需要添加可溶性因子 情况。其中包括：(1)生成特定于 当抗原在肿瘤细胞和反应的脾上时的同种异体抗原 细胞来自未免疫的小鼠；(2)产生特异性CTL 同基因肿瘤抗原当应答的脾细胞来自 未免疫的小鼠；以及(3)从小鼠的前体产生抗病毒的CTL 如果使用某些病毒多肽，则免疫小鼠。 我们已经开始对所需的因素进行生化表征，并已 发现同种异体反应所需的因子可能来自 佛波酯和刀豆蛋白A刺激的ROM4细胞和 未刺激的WEHI-3、AFSTL1和AFSTL2细胞。WEHI-3因子是 白介素3也在辅助产生抗病毒药物方面发挥作用 使用多肽抗原的CTL。 我们的主要目标是提纯和表征来自 ROM4、AFSTL1和AFSTL2细胞在生化和功能上都有。(Hf)

We recently found that the in vitro generation of cytolytic T lymphocytes (CTL) from mouse spleen cells requires added soluble factors under some circumstances. These include the: (1) generation of CTL specific for alloantigens when the antigen is on tumor cells and the responding spleen cells are from nonimmunized mice; (2) generation of CTL specific for syngeneic tumor antigens when the responding spleen cells are from nonimmunized mice; and (3) generation of antiviral CTL from precursors from immunized mice if certain viral peptides are used. We have begun to characterize the needed factors biochemically and have found that the factor needed for the allogeneic response can come from phorbol myristic acetate and Concanavalin A-stimulated ROM4 cells and from nonstimulated WEHI-3, AFSTL1 and AFSTL2 cells. The WEHI-3 factor is interleukin-3 which also functions in assisting the generation of antiviral CTL using peptide antigens. Our major objective is to purify and characterize the new factors from ROM4, AFSTL1, and AFSTL2 cells both biochemically and functionally. (HF)