ONTOGENY AND DIFFERENTIATION OF IGE-B LYMPHOCYTES

IGE-B 淋巴细胞的个体发育和分化

• 批准号: 3125885
• 负责人: KIMISHIGE ISHIZAKA
• 金额: $ 11.68万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1986-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3125885
• 关键词: B lymphocyte; T lymphocyte; antibody; cell differentiation; helper T lymphocyte; histogenesis; humoral immunity; immune adherence reaction; immunofluorescence technique; immunoglobulin E; immunoglobulin M; immunopathology; immunoregulation; microorganism antigen; monoclonal antibody; radiotracer; suppressor T lymphocyte; tissue /cell culture; ultracentrifugation

Our previous studies have shown that lymphocytes from rats infected with Nipostrongylus brasiliensis (Nb) formed soluble factors which have affinity for IgE (IgE-binding factor). One of the IgE-binding factors was spontaneously released from lymphocytes obtained 14 days after Nb-infection, and selectively potentiated an in vitro IgE response of rat lymphocytes to an unrelated antigen (IgE-potentiating factor). Another IgE binding factor was formed by incubation with IgE of lymphocytes from the 8th day infected rats, and this factor selectively suppressed the IgE response (IgE-specific suppressive factor). In this proposal, 1) experiments will be carried out to show that the formation of the IgE-binding factors is not restricted to parasite infection. Lymphocytes from rats treated with complete Freund's adjuvant and cells precultured with concavalin A will be incubated for 24 hr in the presence or absence of IgE, and culture filtrates will be assessed for the presence of either IgE-potentiating factor or IgE-specific suppressive factor or both. 2) The physcochemical properties of the IgE binding factors will be studied to elucidate the difference between IgE-potentiating factor and IgE specific suppressive factor. 3) Cellular origin of the two IgE-binding factors will be investigated. 4) Possible relationship between IgE-binding factors and Fc epsilon receptors on lymphocytes will be studied. We believe that both IgE-potentiating factor and IgE-specific suppressive factor are involved in IgE-isotype specific regulation. 5) The role of these factors in the in vivo IgE antibody response will be evaluated.

我们之前的研究表明，感染了猪流感病毒的大鼠的淋巴细胞 巴西拟圆线虫(Nipostrong ylus brasiliens，Nb)形成的可溶性因子具有亲和力 Ige(IgE结合因子)。免疫球蛋白E结合因子之一是自发的 从感染NB14天后获得的淋巴细胞释放，并选择性地 增强大鼠淋巴细胞对一种无关抗原的体外IgE反应 (免疫球蛋白增强因子)。另一种IgE结合因子是通过孵育形成的 与感染第8天大鼠淋巴细胞的IgE和该因子 选择性抑制IgE反应(IgE特异性抑制因子)。在……里面 这项建议，1)将进行实验，以证明形成的 IgE结合因子并不局限于寄生虫感染。淋巴细胞 来自完全弗氏佐剂处理的大鼠和预先培养的细胞 刀豆蛋白A将在有或没有IgE的情况下孵育24小时，以及 将对培养滤液进行评估，以确定是否存在IgE增强 因子或IgE特异性抑制因子或两者兼而有之。2)物理化学 将对IgE结合因子的性质进行研究，以阐明 IgE增强因子和特异性抑制因子的差异。 3)探讨两种IgE结合因子的细胞来源。4) IgE结合因子与Fc-epsilon受体的关系 将对淋巴细胞进行研究。我们认为，IgE增强因子和 免疫球蛋白特异性抑制因子参与了免疫球蛋白E同型特异性调节。 5)这些因素在体内IgE抗体反应中的作用将是 已评估。