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Next generation transgenic technologies for the chick

下一代小鸡转基因技术

基本信息

批准号：
BB/H023593/1
负责人：
Helen Sang
金额：
$ 15.32万
依托单位：
University of Edinburgh
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2010
资助国家：
英国
起止时间：
2010 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FH023593%2F1
关键词：
Next generation transgenic technologies chick

项目摘要

The chick embryo is a well-established model for the study of vertebrate development, particularly because it is possible to access the embryos in ovo and carry out experimental manipulations that include grafting of embryonic material, application of growth factors, transient transfection with gene constructs by electroporation or infection with retroviral vectors. The chicken is also a model species for investigation of the general physiology of birds, for example photoperiodism and seasonality. The domestic fowl is of course an important agricultural species and there is significant research in the UK into aspects of the genetics and physiology of the chicken relevant to poultry breeding and production. A significant tool that was missing from the repertoire of technologies available to researchers studying the chicken was the ability to make genetic modifications. We established an effective method for production of transgenic birds, by injection of lentiviral vectors into chick embryos in new laid eggs, followed by culture of injected embryos to hatch. We are proposing additional development of transgenic tools for genetic modification of the chick. Our aim is to improve the efficiency of production of transgenic birds and demonstrate that we can use novel tools to investigate gene function directly in birds. These advances will support research in basic biology of vertebrate development through to understanding aspects of disease susceptibility and resistance in poultry.

鸡胚胎是研究脊椎动物发育的成熟模型，特别是因为它可以在卵内获得胚胎并进行实验操作，包括胚胎材料的移植、生长因子的应用、通过电穿孔用基因构建体瞬时转染或用逆转录病毒载体感染。鸡也是研究鸟类一般生理学的模式物种，例如光周期性和季节性。家禽当然是一种重要的农业物种，在英国，对与家禽育种和生产相关的鸡的遗传学和生理学方面进行了重要的研究。研究鸡的研究人员可用的技术库中缺少的一个重要工具是进行遗传修饰的能力。我们建立了一种生产转基因鸡的有效方法，通过将慢病毒载体注射到新产蛋的鸡胚中，然后培养注射的胚胎以孵化。我们建议进一步开发用于鸡的遗传修饰的转基因工具。我们的目标是提高生产转基因鸟类的效率，并证明我们可以使用新的工具来研究直接在鸟类中的基因功能。这些进展将支持脊椎动物发育的基础生物学研究，以了解家禽对疾病的易感性和抵抗力。