Clinical applications of recombinant CSF1-Fc produced in the eggs of transgenic hens in veterinary and human medicine

转基因母鸡鸡蛋产生的重组CSF1-Fc在兽医和人类医学中的临床应用

• 批准号: BB/M021920/1
• 负责人: Helen Sang
• 金额: $ 70.65万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM021920%2F1
• 关键词: Clinical; applications; recombinant; CSF1; Fc

Biologics are pharmaceutical proteins very effective at treating a number of previously intractable medical conditions. They are an increasing proportion of the human drugs market, but widespread use in veterinary settings has been prevented by high production cost. Replacing the expensive mammalian cell culture systems with transgenic chickens has significant potential to reduce this cost. With our system, hens can produce protein of consistent quantity and activity over their entire laying lives, and purification is performed using standard techniques. Here we propose to take forward our recent success producing active pig CSF1-Fc in transgenic hens' eggs by purifying large quantities and testing in potential applications. We will scale up purification, perform full quality control, test in pig models of human disease, and provide material to other experts for evaluation. We will also produce a new transgenic line expressing dog CSF1-Fc for future studies in veterinary medicine.

生物制剂是药物蛋白质，在治疗许多以前棘手的医疗条件非常有效。它们在人用药物市场中的比例越来越大，但由于生产成本高，在兽医环境中的广泛使用受到阻碍。用转基因鸡取代昂贵的哺乳动物细胞培养系统，有很大的潜力降低这一成本。使用我们的系统，母鸡可以在整个产蛋期内生产数量和活性一致的蛋白质，并使用标准技术进行纯化。在这里，我们建议继续我们最近的成功生产活性猪CSF 1-Fc的转基因鸡蛋，通过纯化大量和测试在潜在的应用。我们将扩大纯化规模，进行全面的质量控制，在人类疾病的猪模型中进行测试，并为其他专家提供材料进行评估。我们还将产生一个新的转基因株系表达犬CSF 1-Fc，用于未来的兽医学研究。

项目成果

Additional file 2: of A chicken bioreactor for efficient production of functional cytokines

附加文件2：高效生产功能性细胞因子的鸡生物反应器

• DOI: 10.6084/m9.figshare.7532264
• 发表时间: 2018
• 作者: Herron L

Additional file 3: of A chicken bioreactor for efficient production of functional cytokines

附加文件3：高效生产功能性细胞因子的鸡生物反应器

• DOI: 10.6084/m9.figshare.7532270
• 发表时间: 2018
• 作者: Herron L