NEW MICROBIAL VITAMIN-COENZYME RELATIONSHIPS

新的微生物维生素-辅酶关系

• 批准号: 3125154
• 负责人: RALPH S WOLFE
• 金额: $ 15.6万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1974
• 资助国家: 美国
• 起止时间: 1974-09-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3125154
• 关键词: Archaea; Methanobacteriaceae; analytical chemistry; autoradiography; bacterial genetics; carbon dioxide; cofactor; enzyme mechanism; enzyme structure; growth factor; methyl group; microorganism metabolism; mutant; radiotracer; reduction; stoichiometry; vitamins

The purpose of the proposed research is to elucidate tho biochemistry, physiology, and structure of new coenzymes and vitamins that have been discovered in methanogenic bacteria. In the history of biochemistry, microbes have played a pivotal role in the assay of vitamins as well as in the elucidation of the biochemical function of vitamins and coenzymes. To completely understand cell chemistry is important, for biochemical deficiences produce a diseased state. So far the structures of 6 new coenzymes of methanogens have been documented. It is our goal to define the biochemistry of carbon dioxide reduction to methane, a process which requires the participation of these coenzyme. The major specific aims for this applications are; (i) to study the activation and reduction of carbon dioxide that leads to the formation of a formyl group on the coenzyme, methanofuran, a new method of carbon dioxide fixation; (ii) to study the reduction of a methylene group attached to the coenzyme, tetrahydromethanopterin, and define the products of the reaction; (iii) to define the electron carriers that are involved in these enzymatic reductive reactions; (iv) to study the reductive regeneration of the mercapto form of each of two coenzymes (HS-CoM and 7- mercaptoheptanoylthreonine phosphate) from their heterodisulfide, which is the oxidized product of the terminal methanogenic reaction; (v) to isolate and determine the structure of a vitamin required for the growth of Methanomicrobium mobile. We cannot predict whether these coenzymes will be of chemotherapeutic value in human disease, or whether they will contribute only in a general understanding of biochemistry. The discovery of the archaebacteria is only about a decade old, and since these organisms differ in many ways from typical bacterial, it is important to fully document their biochemistry and identify potential uses to benefit man.

拟议研究的目的是阐明生物化学， 新辅酶和维生素的生理学和结构 在产甲烷细菌中发现。在生物化学史上， 微生物在维生素和维生素的测定中发挥着关键作用 阐明维生素和辅酶的生化功能。 完全理解细胞化学对于生化来说很重要 缺陷会产生疾病状态。到目前为止 6 个新的结构 产甲烷菌的辅酶已被记录。我们的目标是定义 二氧化碳还原为甲烷的生物化学过程 需要这些辅酶的参与。主要具体目标为 该应用程序是； (i) 研究激活和减少 二氧化碳导致在其上形成甲酰基 辅酶，甲烷呋喃，二氧化碳固定的新方法； (二) 至 研究与辅酶相连的亚甲基的还原， 四氢甲烷蝶呤，并定义反应产物； (三) 定义参与这些酶促反应的电子载体 还原反应； (iv) 研究还原再生 两种辅酶（HS-CoM 和 7- 巯基庚酰苏氨酸磷酸盐）从它们的杂二硫化物中分离出来， 是最终产甲烷反应的氧化产物； (五) 至 分离并确定生长所需维生素的结构 甲烷微生物移动。 我们无法预测这些辅酶是否具有化疗作用 对人类疾病的价值，或者它们是否只会在一般情况下做出贡献 对生物化学的了解。古细菌的发现是 只有大约十年的历史，并且由于这些生物体在很多方面都有所不同 从典型的细菌中，完整记录它们是很重要的 生物化学并确定造福人类的潜在用途。