NEW MICROBIAL VITAMIN - COENZYME RELATIONSHIPS

新的微生物维生素-辅酶关系

• 批准号: 3125150
• 负责人: RALPH S WOLFE
• 金额: $ 10.12万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1974
• 资助国家: 美国
• 起止时间: 1974-09-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3125150
• 关键词: Methanobacteriaceae; analytical chemistry; autoradiography; bacterial genetics; biochemistry; carbon dioxide; cofactor; enzyme mechanism; enzyme structure; formaldehyde; growth factor; high performance liquid chromatography; mass spectrometry; microorganism metabolism; nuclear magnetic resonance spectroscopy; nutrition related tag; radiotracer; reduction; scintillation counter; sulfonate; thin layer chromatography; vitamins

The purpose of the propsed research is to elucidate the biochemistry, physiology, and structure of new coenzymes and vitamins that have been discovered in methanogenic bacteria. In the history of biochemistry, microbbes have played a pivitol role in the assay of viatamins as well as in the elucidation of the biochemical function of vitamins and coenzymes. To completely understand cell chemistry is important, for biochemical deficiencies produce a diseased state. Study of methanogens have revealed 7 new factor-coenzyme relationships. Study of these coenzymes is providing new insights into biochemical reactions. To study these coenzymes methanogens are mass cultered in kilogram quantities on H2 + CO2. Coenzymes are frationated from cell extracts to homogeniety, and their structure is then determined by use of mass spectrometry, 1H-NMR, 13C-NMR, elemental analysis and other analytical procedures. The biochemical role of each coenzyme in enzymatic catalysis is studied theough purification of the enzymes that requires the coenzyme for catalytic activity. Methanogens are only distantly related to typical bacteria, and because of their unusual metabolism are providing unique handles by processing certain coenzymes in unusually high concentrations.

这项研究的目的是阐明生物化学， 生理学和新的辅酶和维生素的结构， 在产甲烷细菌中发现。 在生物化学史上， 微生物在维生素A的测定中发挥了pivitol的作用， 在阐明维生素和辅酶的生物化学功能方面。 要完全理解细胞化学是很重要的， 缺陷产生疾病状态。 对产甲烷菌的研究表明 7种新的因子-辅酶关系。 对这些辅酶的研究提供了 对生化反应的新见解 研究这些辅酶 在H2 + CO2上以千克量大量培养产甲烷菌。 辅酶是从细胞提取物中分离出来的， 然后通过使用质谱、1H-NMR、13 C-NMR 元素分析和其他分析方法。 生物化学的作用 研究了酶促反应中各辅酶的纯化方法， 需要辅酶来发挥催化活性的酶。 甲烷菌 与典型的细菌只有远亲关系， 不寻常的新陈代谢提供了独特的处理， 高浓度的辅酶