GENETIC RECOMBINATION IN BACILLUS SUBTILIS

枯草芽孢杆菌的基因重组

• 批准号: 3124688
• 负责人: DAVID A DUBNAU
• 金额: $ 10.31万
• 依托单位: PUBLIC HEALTH RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124688
• 关键词: Bacillus subtilis; DNA; autoradiography; bacterial genetics; binding proteins; cell transformation; clone cells; gel electrophoresis; gene expression; genetic manipulation; genetic mapping; genetic recombination; genetic transcription; host organism interaction; microorganism genetics; mutant; nucleic acid hybridization; nucleic acid sequence; plasmids; protein biosynthesis; radiotracer; transposon /insertion element

Genetic transformation in Bacillus subtilis is a process by which DNA is taken up and integrated into a homologous recipient genome, thereby permanently altering the heredity of the cell. Transformation in this organism is dependent on the development of the competent state. Competence is reached during the stationary phase of growth in specific media. Thus, the development of "natural" competence is distinct from competence in Escherichia coli. It involves the synthesis of several proteins and is accompanied by the cessation of DNA replication and the synthesis of stable RNA. This project will characterize the molecular events that occur during the binding, fragmentation, and uptake of donor transforming DNA. The physical state of the recipient genome in the competent cell and during transformation will also be characterized. An existing collection of transposon-induced non- competent mutants will be investigated. The competence genes defined by these mutations will be cloned and sequenced. The products of these genes will be characterized. The regulation of competence gene expression will be investigated, particularly with regard to the level at which regulation takes place, the interactions of competence genes, and the sigma factors required for their expression. The possibility that competent cells produce a soluble, extracellular factor required for the development of competence will be investigated. Additional competence deficient mutants will be isolated and characterized, in an attempt to approach saturation of the competence gene map.

枯草芽孢杆菌中的遗传转化是一个过程， DNA被吸收并整合到同源受体中 基因组，从而永久改变细胞的遗传。 这种生物体的转化依赖于 有能力的国家。 能力是在 在特定培养基中生长的稳定期。 因此 “自然”能力的发展不同于能力 在大肠杆菌中。 它涉及到几种蛋白质的合成 并伴随着DNA复制的停止， 合成稳定的RNA。 这个项目将描述发生在 在供体的结合、片段化和摄取期间， 转化DNA 受体基因组的物理状态 感受态细胞和转化期间也将 表征了 现有的转座子诱导的非- 将研究有能力的突变体。 能力基因 将被克隆并测序。 的 这些基因的产物将被表征。 调控 感受态基因的表达将被研究，特别是 关于进行监管的级别， 能力基因的相互作用，以及所需的sigma因子 因为他们的表达。 感受态细胞产生 一种可溶性的细胞外因子， 能力将受到调查。 额外权限 缺陷突变体将被分离和表征， 试图接近感受态基因图谱的饱和。