Genetic Competence Apparatus of Bacillus Subtilis

枯草芽孢杆菌遗传能力仪

• 批准号: 8646922
• 负责人: DAVID A DUBNAU
• 金额: $ 39.75万
• 依托单位: RBHS-NEW JERSEY MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-12-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8646922
• 关键词: ATP phosphohydrolase; Accounting; Antibiotic Resistance; Bacillus subtilis; Bacteria; Binding; Biochemical Genetics; Biochemistry; Cell Wall; Cell division; Cell surface; Cells; Cellular biology; Chromosomes; Competence; Complex; Cytokinesis; DNA; DNA Binding; DNA biosynthesis; DNA receptor; DNA-Binding Proteins; Development; Environment; Enzymes; Event; Evolution; Face; Fimbriae Proteins; Fluorescent in Situ Hybridization; Funding; Gene Exchanges; Gene Targeting; Genes; Genetic; Genetic Recombination; Genetic Screening; Grant; Growth; Horizontal Gene Transfer; Hour; Imagery; In Vitro; Inherited; Learning; Lifting; Membrane; Methods; Microscopic; Microscopy; Minor; Modeling; Motor; Operon; Orthologous Gene; Peptide Hydrolases; Phase; Physiological; Pilum; Process; Proteins; RNA; Role; Route; Single-Stranded DNA; Site; Speed; Structure; Surface; System; Testing; Time; Virulence; Work; cell fixation; daughter cell; homologous recombination; knockout gene; nuclease; prevent; public health relevance; recombinational repair; research study; single molecule; tool; trafficking; uptake

DESCRIPTION (provided by applicant): In bacteria, genetic information is transferred "vertically" because DNA is inherited by daughter cells. Genetic information can also be transferred "horizontally", accounting in many cases for the exchange of genes that contribute to virulence and antibiotic resistance. Horizontal transfer is a major factor in the evolution of bacteria. An important route of horizontal transfer is by the release of DNA into the environment and the subsequent uptake of this DNA by bacteria, a process known as transformation. The work proposed in this application is devoted to obtaining a mechanistic understanding of the transformation process. We wish to learn how cells bind DNA to their surface and how they internalize this DNA, the role of a filamentous structure known as the pseudopilus in the uptake process and to use microscopy to visualize the uptake and processing of the DNA once it is internalized as well as the interaction of the DNA with the recipient cell chromosome. Cells that are capable of being transformed are in a physiological state in which their growth, division and DNA replication are halted. We will continue our efforts to understand the causes of this arrest and the mechanisms that enable the cells to eventually resume growth.

描述（由申请人提供）：在细菌中，遗传信息是“垂直”转移的，因为DNA是由子细胞继承的。遗传信息也可以“横向”转移，在许多情况下，导致致病性和抗生素耐药性的基因交换。水平转移是细菌进化的主要因素。水平转移的一个重要途径是将DNA释放到环境中，随后细菌吸收该DNA，这一过程称为转化。本申请中提出的工作致力于获得对转化过程的机械理解。我们希望了解细胞如何将DNA结合到它们的表面以及它们如何内化这种DNA，被称为假纤毛的丝状结构在摄取过程中的作用，并使用显微镜观察DNA一旦内化后的摄取和加工以及DNA与受体细胞染色体的相互作用。能够被转化的细胞处于其生长、分裂和DNA复制停止的生理状态。我们将继续努力了解这种停滞的原因以及使细胞最终恢复生长的机制。