Genetic competence apparatus of Bacillus subtilis

枯草芽孢杆菌遗传能力装置

• 批准号: 7753893
• 负责人: DAVID A DUBNAU
• 金额: $ 38.61万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-12-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7753893
• 关键词: Address; Antibiotic Resistance; Automobile Driving; Bacillus subtilis; Bacteria; Biochemistry; Cell surface; Cells; Chromosomes; Competence; Complex; DNA; DNA Binding; DNA-Binding Proteins; Energy-Generating Resources; Environment; Funding; Gene Transfer; Genes; Genetic; Genetic Screening; Hand; Light; Membrane; Molecular; Physiological; Pilum; Process; Processed Genes; Progress Reports; Proteins; Protocols documentation; Published Comment; Reagent; Role; Single-Stranded DNA; Site; Staging; Structure; Study Section; Testing; Text; Update; Virulence; base; design; insight; nuclease; pathogen; research study

DESCRIPTION (provided by applicant): Bacillus subtilis and many other bacteria can be transformed by exogenous DNA when in a physiological state known as competence. Competent cells can bind DNA, fragment it on the cell surface and transport a single strand of DNA across the membrane. Once internalized, the single strand can integrate at a homologous site on the recipient chromosome. Our long-term objective is to understand these processes on the molecular level, by characterizing the 20 proteins involved and their interactions with one another and with DNA. We have shown that transformation takes place mostly at the cell poles. This proposal explores the interactions among many of the competence proteins, as well as their localization and delocalization as polar complexes. The recently discovered role of McsA and McsB in delocalization will be explored. We will also detect contacts between these proteins and DNA at various stages in the transformation process and determine the cellular addresses at which the contacts occur. The structure, composition and role of the competence pseudopilus will be explored. The sources of energy driving the transport of transforming DNA will be identified. Genetic screens will be used to find two competence proteins that have not been identified; the nuclease that degrades the non-transforming strand, and a second cell surface DNA binding protein. Transformation provides a mechanism for the horizontal transfer of antibiotic resistance and virulence genes among pathogens. Understanding the mechanism of gene transfer by transformation will provide insights into these important processes. Many bacteria can accept DNA from the environment in a process known as transformation. This provides a mechanism for the horizontal transfer of antibiotic resistance and virulence genes among pathogens. Understanding this process of gene transfer by transformation will provide insights into these important processes.

描述（由申请人提供）：枯草芽孢杆菌和许多其他细菌在处于称为能力的生理状态时可以被外源DNA转化。感受态细胞可以结合 DNA，在细胞表面将其片段化，并将单链 DNA 跨膜运输。一旦内化，单链可以整合到受体染色体上的同源位点。我们的长期目标是通过表征所涉及的 20 种蛋白质及其彼此之间以及与 DNA 的相互作用，在分子水平上了解这些过程。我们已经证明转化主要发生在细胞两极。该提案探讨了许多能力蛋白之间的相互作用，以及它们作为极性复合物的定位和离域。我们将探讨最近发现的 McsA 和 McsB 在离域中的作用。我们还将在转化过程的各个阶段检测这些蛋白质和 DNA 之间的接触，并确定发生接触的细胞地址。将探讨拟菌毛能力的结构、组成和作用。驱动转化 DNA 运输的能量来源将被确定。遗传筛选将用于寻找两种尚未鉴定的能力蛋白；降解非转化链的核酸酶和第二种细胞表面 DNA 结合蛋白。转化提供了抗生素抗性和毒力基因在病原体之间水平转移的机制。了解通过转化进行基因转移的机制将有助于深入了解这些重要过程。许多细菌可以通过称为转化的过程从环境中接受 DNA。这为病原体之间抗生素抗性和毒力基因的水平转移提供了机制。了解通过转化进行基因转移的过程将有助于深入了解这些重要过程。