BIACORE 2000

此应用程序是一个Biacore 2000仪器位于 公共卫生研究所。 一组五名校长 研究人员，他们都是NIH赠款的接受者，将组成 主要用户群。 此外， 研究所将有选择地使用该仪器。的 将应用Biacore仪器的项目各不相同， 自然其中两人（品特博士和蒂利博士）与艾滋病毒有关。品特医生 关注疫苗开发，并将利用SPR探索 各种单克隆抗体对V1/V2结构域中确定的表位的亲和力。 博士蒂利将使用该仪器研究 单克隆抗体和HIV-1包膜糖蛋白血清阳性血清， 其表位。 Penetsky博士将使用SPR来探索 F1/F0 ATP酶。Dubnau博士将使用Biacore 2000来研究 蛋白质-蛋白质和蛋白质-DNA相互作用的调节 的能力发展和转化DNA的吸收能力 细菌派恩博士将探讨M。结核感染 巨噬细胞的DNA结合活性的几个转录 因素

This application is for a Biacore 2000 Instrument to be located at the Public Health Research Institute. A group of five principal investigators, all of whom are recipients of NIH grants, will comprise the major user group. In addition, minor users both inside and outside the Institute will have access to the instrument on a selective basis. The projects to which the Biacore instrument will be applied are varied in nature. Two of them (Drs. Pinter and Tilley) are HIV -related. Dr. Pinter is concerned with vaccine development and will use SPR to explore the affinities of various monoclonals to defined epitopes in the V1/V2 domain. Dr. Tilley will use the instrument to study the reactivities of monoclonals, and seropositive sera with HIV-1 envelope glycoproteins and epitopes thereof. Dr. Penetsky will use SPR to explore the assembly of the F1/F0 ATPase. Dr. Dubnau will use the Biacore 2000 to study a variety of protein-protein and protein-DNA interactions involved in the regulation of competence development and the uptake of transforming DNA by competent bacteria. Dr. Pine will explore the influence of M. tuberculosis infection of macrophages on the DNA-binding activities of several transcription factors.