BIACORE 2000

比亚科2000

• 批准号: 2472071
• 负责人: DAVID A DUBNAU
• 金额: $ 22万
• 依托单位: PUBLIC HEALTH RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2472071
• 关键词: biomedical equipment; biomedical equipment purchase; biosensor device; intermolecular interaction

This application is for a Biacore 2000 Instrument to be located at the Public Health Research Institute. A group of five principal investigators, all of whom are recipients of NIH grants, will comprise the major user group. In addition, minor users both inside and outside the Institute will have access to the instrument on a selective basis. The projects to which the Biacore instrument will be applied are varied in nature. Two of them (Drs. Pinter and Tilley) are HIV -related. Dr. Pinter is concerned with vaccine development and will use SPR to explore the affinities of various monoclonals to defined epitopes in the V1/V2 domain. Dr. Tilley will use the instrument to study the reactivities of monoclonals, and seropositive sera with HIV-1 envelope glycoproteins and epitopes thereof. Dr. Penetsky will use SPR to explore the assembly of the F1/F0 ATPase. Dr. Dubnau will use the Biacore 2000 to study a variety of protein-protein and protein-DNA interactions involved in the regulation of competence development and the uptake of transforming DNA by competent bacteria. Dr. Pine will explore the influence of M. tuberculosis infection of macrophages on the DNA-binding activities of several transcription factors.

此申请是为了让Biacore 2000仪器位于 公共卫生研究所。由五名校长组成的小组 调查人员，他们都是NIH拨款的接受者，将包括 主要用户群。此外，内部和外部的次要用户 研究所将有选择地使用该仪器。这个 将应用Biacore工具的项目各不相同 大自然。其中两人(品特博士和蒂利博士)与艾滋病毒有关。品特博士 关注疫苗开发，并将使用SPR来探索 不同单克隆体与V1/V2结构域中已定义表位的亲和力。 Tilley博士将使用该仪器来研究 具有HIV-1包膜糖蛋白的单克隆和血清阳性血清 它们的表位。佩内茨基博士将使用SPR探索组装 F1/F0 ATPase。杜布诺博士将使用Biacore 2000研究一种 参与调控的蛋白质-蛋白质和蛋白质-DNA相互作用 能力发展和能力对转化DNA的吸收 细菌。派恩博士将探讨结核分枝杆菌感染的影响 巨噬细胞对几种转录因子DNA结合活性的影响 各种因素。