PRODUCTION AND ANALYSIS OF HISTOCOMPATIBILITY MUTANTS

这个项目致力于维持、发展和扩大一群老鼠 携带影响皮肤移植的基因突变。一个 相当数量的线路已经在建、完工或正在建设中 发展。这些菌株包括携带H-2突变的菌株以及 非H-2基因突变。将通过筛选新的突变来寻找 经ENU处理和未处理的C57BL/6Kh和C57BL/6Kh品系雄性小鼠的后代 Balb/CKH。此外还有一些其他自交系、同源和重组 菌株将被持续筛选，作为系统的一部分，以监测它们的 遗传完整性和突变也可能从它们身上恢复。 突变者将进入免疫遗传分析，两者都是正式的 合作努力，并将其提供给其他调查人员 如有要求，请提供。正式合作的包括斯坦利·内森森博士 突变基因产物在蛋白质上的生化和结构分析 和DNA水平)，Ian McKenzie博士(突变基因的血清学分析 产品)，Kees Melef博士(突变的功能后果 CML、MLR和MHC限制等细胞介导的分析)，Peter博士 Wettstein(寻找整合病毒序列的变化 涉及非H-2基因突变的突变)，以及Bung Kim博士 (协助电泳法)。 我们将专门检查最近重新出现的H-2dm6突变 (它失去了DD，但保留了LD，显然也保留了RD)，看看D 除D、L和R外的区域产品可被确定为 变种人。我们还建议尝试用同种异体抗血清来对抗一些 作为未来分析组织相容性突变的一步 这些基因。

This project seeks to maintain, to develop and to expand a colony of mice which carry mutations of genes which affect skin graft transplantation. A substantial number of lines are already on hand, completed or under development. These include strains bearing H-2 mutations as well as non-H-2 gene mutations. New mutations will be sought by screening the progeny of ENU-treated or untreated male mice of strains C57BL/6Kh and BALB/cKh. In addition a number of other inbred, congenic and recombinant strains will be continually screened as part of a system to monitor their genetic integrity and mutants may be recovered from them as well. Mutants will be entered into immunogenetic analyses, both as part of formal collaborative efforts and by making them available to other investigators upon request. The formal collaborations include Dr. Stanley Nathenson (biochemical and structural analysis of mutant gene products at the protein and DNA levels), Dr. Ian McKenzie (serological analyses of mutant gene products), Dr. Kees Melief (functional consequences of the mutations for cell-mediated assays such as CML, MLR and MHC restriction), Dr. Peter Wettstein (looking for changes in integrated viral sequences as a result of mutations involving mutations of non-H-2 genes), and Dr. Byung Kim (assisting in electrophoretic techniques). We will be specifically examining the recently reovered H-2dm6 mutation (which has lost Dd, but retained Ld and apparently Rd as well) to see if D region products other than D,L and R can be identified which are missing in the mutant. We also propose attempting to raise alloantisera against some of the minor histocompatibility mutants as a step toward future analyses of these genes.