MOLECULAR EVENTS IN ANTIGEN RECOGNITION

这项建议主要有两个方面的调查：1)检查B细胞 抗原递呈给T细胞和2)表征抗原处理 巨噬细胞和B细胞。利用功能研究，我们将研究 B细胞向T细胞递呈抗原性洗涤剂的能力 类似于巨噬细胞。我们将确定B细胞是否呈现出 T细胞的抗原受MHC限制。此外，我们还将测试 小鼠淋巴瘤细胞和新生儿B细胞提呈抗原的能力 T细胞。利用功能、生化和免疫化学技术，我们 将研究巨噬细胞的物理和免疫学特性 B细胞抗原处理。定性将包括研究 抗原降解和溶酶体参与抗原加工。 使用完整的巨噬细胞和B细胞以及亚细胞部分 我们将研究这些细胞的免疫活性特征 加工抗原及其与MHC基因产物的可能关联。

This proposal has two main areas of investigation: 1) to examine B cell antigen presentation to T cells and 2) to characterize antigen processing by macrophages and B cells. Using functional studies, we will examine the ability of B cells to present antigenic determiants to T cells in a manner similar to macrophages. We will determine if B cell presentation of antigen to T cells is MHC restricted. In addition, we will test the ability of murine lymphoma cells and neonatal B cells to present antigen to T cells. Using functional, biochemical and immunochemical techniques we will study the physical and immunological characteristics of macrophage and B cell antigen processing. Characterization will include studies of antigen degradation and the involement of lysosomes in antigen processing. Using both intact macrophages and B cells and subcellular fractions of these cells we will study the characteristics of immunologically active processed antigen and its possible association with MHC gene products.