MOLECULAR EVENTS IN ANTIGEN RECOGNITION

该建议有两个主要的研究领域：1）检查B细胞 抗原呈递给T细胞和2）表征抗原加工 由巨噬细胞和B细胞产生。 使用功能研究，我们将检查 B细胞向T细胞呈递抗原决定物的能力， 类似于巨噬细胞。 我们将确定是否B细胞呈递 T细胞抗原是MHC限制性的。 此外，我们将测试 小鼠淋巴瘤细胞和新生儿B细胞呈递抗原的能力 T细胞。 利用功能、生物化学和免疫化学技术， 将研究巨噬细胞的物理和免疫特性， B细胞抗原处理。 表征将包括以下研究： 抗原降解和溶酶体参与抗原加工。 使用完整的巨噬细胞和B细胞以及 我们将研究这些细胞的免疫活性特征， 加工抗原及其与MHC基因产物的可能关联。

This proposal has two main areas of investigation: 1) to examine B cell antigen presentation to T cells and 2) to characterize antigen processing by macrophages and B cells. Using functional studies, we will examine the ability of B cells to present antigenic determiants to T cells in a manner similar to macrophages. We will determine if B cell presentation of antigen to T cells is MHC restricted. In addition, we will test the ability of murine lymphoma cells and neonatal B cells to present antigen to T cells. Using functional, biochemical and immunochemical techniques we will study the physical and immunological characteristics of macrophage and B cell antigen processing. Characterization will include studies of antigen degradation and the involement of lysosomes in antigen processing. Using both intact macrophages and B cells and subcellular fractions of these cells we will study the characteristics of immunologically active processed antigen and its possible association with MHC gene products.