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MICROBIOLOGY AND PHYSIOLOGY OF METHANOGENESIS

产甲烷作用的微生物学和生理学

基本信息

批准号：
3129779
负责人：
MEYER J WOLIN
金额：
$ 8.58万
依托单位：
NYSDOH/HEALTH RESEARCH, INC.
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-03-01 至 1987-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3129779
关键词：
Methanobacteriaceae anaerobic bacteria antibody dietary fiber enteric bacteria fatty acid metabolism fermentation gastrointestinal absorption /transport host organism interaction hydrogen large intestine microorganism metabolism nutrition related tag

项目摘要

The goal is to use the rat as a model for studying factors that may influence the establishment of methanogenesis in humans and the significance of methanogenesis to the physiology of the host. Methanogenic bacteria of Wistar and other strains of rats will be isolated and characterized. Methanogens and total viable anaerobes will be concurrently enumeratd in rats on low, normal and high fiber diets and the influence or the diets on the production of CH4, H2 and volatile fatty acids will be measured. Establishment of methanogenic bacteria as a function of age an sex will be investigated. Development of antibodies to autochthonous methanogens will be studied and rats will be immunized with autochthonous strains to determine if antibodies cause elimination of methanogens from the intestine. Non-methanogenic rat strains will be manipulated by varying diets and inoculation with flora from methanogenic strains to see if methanogenesis can be established. Experiments designed to measure the relative contribution of dietary and endogenous substrates to large intestine microbial fermentation are proposed. These involve the use of a basal synthetic diet with nutrients that are completely absorbed in the small intestine. Total CH4 and H2 production as well as the rate of dilution of labeled acetate added to the cecum will be used to measure the contribution of endogenous substrates to fermentation. Identical measurements will be made after additions of substrates that escape monogastric digestion to determine their addtional contribution ot fermentation. The results should indicate why CH4 is a product of large intestine fermentation in 1/3 to 1/2 of normal human adults and not in the rest of the population. Evaluation of the contribution of endogenous substrates to fermentation may provide a basis for using measurements of H2 and CH4 production to evaluate differences in the contribution of endogenous substrates to fermentation in different individuals.

我们的目标是用老鼠作为模型来研究可能影响人类甲烷生成的建立，产甲烷对宿主生理的重要性。产甲烷将分离Wistar和其他品系大鼠的细菌，表征了产甲烷菌和总活厌氧菌将同时在低纤维、正常纤维和高纤维饮食的大鼠中计数，日粮对CH 4、H2和挥发性脂肪酸产生的影响测定了产甲烷细菌的建立与年龄和环境的关系性将被调查。产生抗本地将研究产甲烷菌，并将用本地产甲烷菌免疫大鼠。菌株，以确定抗体是否导致甲烷菌从肠子非产甲烷大鼠品系将通过改变饮食和接种产甲烷菌株的植物群，以观察可以建立甲烷生成。旨在测量膳食和营养素的相对贡献的实验大肠微生物发酵的内源性底物是提出了这些包括使用基础合成饮食与营养在小肠中被完全吸收。 CH 4和H2总量生产以及添加到盲肠将用于测量内源性底物对发酵在添加以下物质后，将进行相同的测量：逃脱单胃消化的底物，以确定它们的附加发酵的贡献。结果应该表明为什么CH 4是大肠的产物发酵在1/3至1/2的正常成人，而不是在其余的人口。评价内源性底物对发酵可以为使用H2和CH 4的测量提供基础生产的差异，以评估内生发酵底物在不同的个人。