IMMUNOCHEMICAL STUDIES OF CHEMOATTRACTANT SUBSTANCES

化学吸引物质的产生被认为起着重要的作用 在感染和过敏过程的发展和结果中的作用。 最近的研究已经引起了人们对化学吸引活性的关注。 LPC(溶血磷脂酰胆碱)、HETES(5-羟基或 12-羟基二十碳四烯酸)和白三烯 膜磷脂。因此，我们发起了一项免疫学研究 和生化调查，目的是：(1)进一步进行 新发现的抗癌药物溶血磷脂酰胆碱的研究 淋巴细胞，以及(2)研究补体诱导的新方面 通过评估补体蛋白C5b-9在炎症中的作用来研究炎症 产生LPC、HETE、白三烯，可能还有其他 膜磷脂中的化学诱导剂。 关于LPC，我们想要确定哪些细胞类型响应其 引诱活性，它在体内扮演着什么样的中介作用 炎症，LPC的哪些结构类似物显示出趋化作用 活性，LPC是否通过反应转化为其他化学诱导剂 细胞，以及LPC是否能诱导新的趋化物质的形成 包括一些具有已知化学诱导剂的花生四烯酸代谢物 活动。关于补体，我们想确定什么类型的细胞 在C5b-9攻击时产生趋化因子并评估 LPC、HETE、白三烯的形成，可能还有其他 化学诱导剂。我们预计，这些努力将扩大对 补体引起的炎症超越了众所周知的 补体片段C3a、C4a、C5a和C3b。我们认为，激活 感染、过敏和恶性肿瘤组织部位的补体很可能 导致膜损伤，从而激活膜相关的 磷脂酶和其他在生产过程中起作用的酶 化学诱导剂磷脂衍生物，包括LPC。

Generation of chemoattractant substances is believed to play an important role in the development and outcome of infectious and allergic processes. Recent studies have attracted attention to chemoattractant activities of substances such as LPC (lysophosphatidylcholine), HETEs (5-hydroxy or 12-hydroxy eicosatetraeonic acid) and leukotrienes which are derived from membrane phospholipids. Accordingly, we have initiated an immunological and biochemical investigation with the purpose of: (1) Conducting further studies of lysophosphatidylcholine, a newly recognized chemoattractant for lymphocytes, and (2) investigating a new aspect of complement-induced inflammation by assessing the role of complement proteins C5b-9 in the generation of LPC, HETEs, leukotrienes and, possibly, other chemoattractants from membrane phospholipids. With respect to LPC we want to determine what cell types respond to its attractant activity, what role it plays in vivo as a mediator of inflammation, what structural analogs of LPC exhibit chemoattractant activity, whether LPC is converted to other chemoattractants by responding cells, and whether LPC can induce formation of new chemoattractants including some arachidonate metabolites with known chemoattractant activity. With respect to complement, we want to determine what cell types produce chemoattractant factors when attacked by C5b-9 and to assess formation of LPC, HETEs, leukotrienes and, possibly, other chemoattractants. We expect that these efforts will expand knowledge of complement-induced inflammation beyond the well-known activities of complement fragments C3a, C4a, C5a, and C3b. We believe that activation of complement at tissue sites of infection, allergy and malignancy, is likely to lead to membrane damage which could activate membrane-associated phospholipase and other enzymes that are instrumental in producing chemoattractant phospholipid derivatives, including LPC.