PLATELET-MEDIATED CYTOTOXICITY:MECHANISMS & APPLICATIONS

我们的长期目标是阐明参与的机制， 免疫系统对肿瘤细胞的破坏。 这 应用的重点是细胞毒性介导的机制 通过血小板。 血小板参与破坏 抗体致敏的靶细胞如红细胞、肿瘤细胞 而棘体幼虫直到最近才被发现。 我们 预计血小板介导的细胞毒性研究将增加 细胞介导的细胞毒性机制的新信息 涉及从细胞中产生细胞毒性因子， 膜，并将促进该反应在 肿瘤疾病的治疗。 具体的调查领域包括：（1）获得额外的 证明了整个分子组合体 识别并裂解血小板血浆中的靶细胞 膜的 (2)功能性表征靶病变 膜。 (3)通过电子显微镜研究 血小板和各种目标之间的联系。 (4)表征和 分离所述裂解分子。 (5)识别分子 参与靶细胞的识别和 裂解分子。 (6)比较的机制 小鼠和人血小板的红细胞溶解。 (7)研究 血小板介导的肿瘤细胞溶解机制。 (8)探索 血小板作为细胞毒性药物或试剂载体的用途， 增强血小板介导的对特定肿瘤细胞靶点的杀伤。

Our long term goal is to elucidate the mechanisms involved in the destruction of tumor cells by the immune system. This application focuses on the mechanisms of cytotoxicity mediated by platelets. The participation of platelets in the destruction of antibody-sensitized targets such as erythrocytes, neoplastic cells and schistosomal larvae has been appreciated only recently. We expect that the study of platelet-mediated cytotoxicity will add new information on the mechanisms of cell-mediated cytotoxicity involving the generation of cytotoxic factor(s) from the membranes and will facilitate the use of this reaction in the treatment of neoplastic diseases. The specific areas to investigate involve: (1) Obtaining additional evidence that the entire molecular assembly capable of recognizing and lysing target cells resides in the platelet plasma membrane. (2) Characterizing functionally the lesion in target membranes. (3) Studying by electron microscopy the interaction between platelets and various targts. (4) Characterizing and isolating the lytic molecule(s). (5) Identifying the molecules involved in the recognition of target cells and in the generation of the lytic molecule(s). (6) Comparing the mechanisms of erythrocyte lysis by mouse and human platelets. (7) Studying the mechanisms of platelet-mediated tumor cell lysis. (8) Exploring the use of platelets as a carrier of cytotoxic drugs or agents to enhance platelet-mediated killing of specific tumor cell targets.