PLATELET-MEDIATED CYTOTOXICITY:MECHANISMS & APPLICATIONS

我们的长期目标是阐明 免疫系统对肿瘤细胞的破坏。这 细胞毒作用机制的应用研究进展 通过血小板。血小板膜破坏中的血小板参与 抗体致敏的靶点，如红细胞、肿瘤细胞 而血吸虫幼虫直到最近才得到重视。我们 预计对血小板介导的细胞毒性的研究将增加 细胞介导的细胞毒作用机制的新进展 涉及细胞毒因子(S)的产生 膜，并将促进该反应在 肿瘤疾病的治疗。 要调查的具体领域涉及：(1)获得额外的 有证据表明，整个分子组装能够 识别和裂解靶细胞驻留在血小板血浆中 薄膜。(2)靶区病变的功能特征 膜。(3)用电子显微镜研究相互作用。 在血小板和各种靶标之间。(4)刻画和 分离裂解分子(S)。(5)分子鉴定 参与识别目标细胞和产生 裂解分子(S)。(6)比较两种方法的作用机理。 小鼠和人血小板裂解红细胞。(七)研究 血小板介导的肿瘤细胞溶解机制。(八)探索 使用血小板作为细胞毒性药物或药物的载体 增强血小板介导的特定肿瘤细胞靶点的杀伤作用。

Our long term goal is to elucidate the mechanisms involved in the destruction of tumor cells by the immune system. This application focuses on the mechanisms of cytotoxicity mediated by platelets. The participation of platelets in the destruction of antibody-sensitized targets such as erythrocytes, neoplastic cells and schistosomal larvae has been appreciated only recently. We expect that the study of platelet-mediated cytotoxicity will add new information on the mechanisms of cell-mediated cytotoxicity involving the generation of cytotoxic factor(s) from the membranes and will facilitate the use of this reaction in the treatment of neoplastic diseases. The specific areas to investigate involve: (1) Obtaining additional evidence that the entire molecular assembly capable of recognizing and lysing target cells resides in the platelet plasma membrane. (2) Characterizing functionally the lesion in target membranes. (3) Studying by electron microscopy the interaction between platelets and various targts. (4) Characterizing and isolating the lytic molecule(s). (5) Identifying the molecules involved in the recognition of target cells and in the generation of the lytic molecule(s). (6) Comparing the mechanisms of erythrocyte lysis by mouse and human platelets. (7) Studying the mechanisms of platelet-mediated tumor cell lysis. (8) Exploring the use of platelets as a carrier of cytotoxic drugs or agents to enhance platelet-mediated killing of specific tumor cell targets.