DELETION MAPPING OF HUMAN CELL SURFACE ANTIGENS

人类细胞表面抗原的缺失图谱

• 批准号: 3127401
• 负责人: DONALD A PIOUS
• 金额: $ 12.6万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-04-01 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3127401
• 关键词: chromosome deletion; chromosomes; gene expression; genetic manipulation; genetic mapping; histocompatibility antigens; human tissue; hybridomas; immunochemistry; major histocompatibility complex; molecular cloning; monoclonal antibody; mutant; tissue /cell culture

The objectives of this research are: 1) to determine the order of the HLA-D genes and complement components B(f) and C4 in the HLA-D region; 2) to map genes coding for T-cell specific antigens, including the putative T cell antigen receptor and genes coding for cell surface antigens linked to the Beta2-microglobulin gene; and 3) to identify and map genes coding for previously unmapped or undetected antigens in these regions. The overall approach will be to isolate deletion variants affecting cell surface antigens of T and B lymphoid cell lines by mutagenesis and immunoselection with appropriate antibodies. Using cloned cDNAs and available monoclonal antibodies, as well as monoclonal antibodies produced in our laboratory, we will use the patterns of reactivity on progenitor cells and their congenic deletion variants to map the genes to the deleted regions, employing methods of analysis worked out in the initial grant period.

本研究的目的是：1）确定 HLA-D基因和HLA-D区的补体成分B（f）和C4; 2） 绘制编码T细胞特异性抗原的基因，包括假定的T细胞抗原， 细胞抗原受体和编码与之连接的细胞表面抗原的基因 β 2-微球蛋白基因;和3）鉴定和定位编码以下的基因： 这些区域中先前未定位或未检测到的抗原。 整体 方法将是分离影响细胞表面的缺失变体 通过诱变和免疫选择的T和B淋巴细胞系的抗原 适当的抗体。 使用克隆的cDNA和可用的单克隆抗体， 抗体以及我们实验室生产的单克隆抗体，我们 将利用祖细胞及其同类细胞的反应模式， 缺失变体以将基因定位到缺失区域，采用 在最初的赠款期间制定的分析方法。