GENETICS OF T-LYMPHOCYTE ANTIGEN RECEPTORS

这项拨款提案旨在更好地了解积极和消极的 塑造T细胞库和一般焦点的选择力 的补助金是对非MHC基因的特征， 胸腺选择 我们计划继续描述一个基因，Et-1， 是I-E反应性细胞克隆缺失所绝对需要的， 其相应的基因产物可能是M1 s家族的成员 抗原。 Et-1与内源性 将确定病毒基因组Mtv-9。 第二I-E共耐受原Et- 2，存在于DBA/2菌株中， 确定是否与内源性病毒有关 序列的 为了研究非MHC基因对正选择的影响， 我们将对背景（非MHC）基因进行分析， 确定V-β-11 +/CD 8 +T细胞的频率。 最后我们将 研究了背景基因对一个具有正选择性的群体的影响， T细胞受体转基因小鼠中单个T细胞受体。

This grant proposal seeks to better understand the positive and negative selective forces which shape the T cell repertoire and the general focus of the grant is on the characterization of non-MHC genes which influence thymic selection. We plan to continue characterizing a gene, Et-1, that is absolutely required for the clonal deletion of I-E reactive cells and whose corresponding gene product is likely a member of the M1s-family of antigens. The molecular relationship between Et-1 and the endogenous viral genome, Mtv-9, will be determined. A second I-E co-tolerogen, Et- 2, present in the DBA/2 strain will be characterized with an emphasis on determining whether or not it is related to endogenous viral sequences. To study the effects of non-MHC genes on positive selection, we will carry out an analysis of the background (non-MHC) genes which determine the frequency of V-beta-11+/CD8+T cells. Finally, we will study the effects of background genes on the positive selection of a single T cell receptor in a T cell receptor transgenic mouse.