Y CHROMOSOME EXPRESSION AND EVOLUTION

本研究的目的是阐明Y 染色体决定性表型。 为此，我们设计了一个 一种特异性克隆Y染色体DNA的新方法， 足够大以包含Y的整个互补序列的文库 染色体DNA序列。 此库将用于分析Y编码 发育中的胚胎中的nRNAs。 这类研究有可能 识别Y染色体上启动转录的基因 性分化的发育途径。 使用这个库 克隆的Y DNA，我们还将研究DNA序列的保守性和漂移 在脊椎动物2亿年的进化过程中， 许多 患有性分化发育障碍的儿童 携带Y染色体的遗传异常 克隆Y染色体 DNA将被用来建立染色体之间的关系 性分化的异常、基因表达和异常模式 在人身上。

The objective of this study is to elucidate the mechanism by which the Y chromosome determines sexual phenotype. To this end, we have devised a novel method for specifically cloning Ychromosome DNA and have constructed a library that is sufficiently large to contain the entire complement of Y chromosome DNA sequences. This library will be used to analyze Y encoded nRNAs in developing embryos. This class of study has the potential to identify transcribed genes on the Y chromosome which initiate the developmental pathway of sexual differentiation. Using this library of cloned Y DNA we will also study the conservation and drift of DNA sequences on the Y chromosome during 200 million years of vertebrate evolution. Many children suffering from developmental disorders of sexual differentiation carry genetic anomalies involving the Y chromosome. Cloned Y chromosome DNA will be used to establish the relationship between chromosome anomalies, gene expression and abnormal patterns of sexual differentiation in man.