THE REGULATION OF V ALPHA 7 EXPRESSING T CELLS

我们已经观察到，表达V α 7基因在C57 BL/10小鼠中的代表性非常低。 这 考虑到V α 7基因占V α的8%-10%， 生殖系基因库 这项拨款中提出的实验 申请寻求确定的机制或过程， 调节细胞中表达V α 7的T细胞的数量， 外围 设计了一组实验来确定V α 7是否 基因携带一种结构缺陷， 或高效转录。 第二组实验被设计来估计频率 胸腺细胞和外周血T细胞中V α 7表达细胞的数量 细胞;这些实验侧重于耐受性的潜在作用 和/或胸腺选择在确定成熟的， 外周V α 7表达T细胞。

We have made the observation that peripheral T cells expressing V alpha7 genes are highly underrepresented in C57BL/10 mice. This is surprising considering V alpha7 genes comprise 8%-10% of V alpha germline gene pool. The experiments proposed in this grant application seek to determine the mechanism or process which regulates the number of V alpha7-expressing T cells in the periphery. A set os experiments is designed to determine whether the V alpha7 genes carry a structural defect that prevents their rearrangement or efficient transcription. A second set of experiments is designed to estimate the frequence of V alpha7-expressing cells among thymocytes and peripheral T cells; these experiments focus on the potential role of tolerance and/or thymic selection in determining the number of mature, peripheral V alpha7-expressing T cells.