LEISHMANIA ANTIGENS-BIOCHEMICAL AND IMMUNOLOGIC STUDIES

利什曼原虫抗原-生化和免疫学研究

• 批准号: 3134810
• 负责人: Diane McMahon Pratt
• 金额: $ 25.12万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1993-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134810
• 关键词: Leishmania; Leishmania donovani; active immunization; affinity chromatography; communicable disease diagnosis; electron microscopy; enzyme linked immunosorbent assay; genetic manipulation; hamsters; high performance liquid chromatography; immunofluorescence technique; immunologic assay /test; immunological substance; ion exchange chromatography; laboratory mouse; laboratory rabbit; leishmaniasis; macrophage; molecular cloning; monoclonal antibody; parasitic disease chemotherapy; parasitic disease diagnosis; passive immunization; protein sequence; protozoal antigen; protozoal vaccine; tissue /cell culture; vaccines

The purpose of this proposal is to continue and extend the previous work supported by grant AI-23004, to delineate both the antigens and mechanisms involved in protective immunity for leishmaniasis. In addition, the proposal is to investigate biochemical differences responsible for the survival of the parasite in each of the life cycle stages (promastigote- amastigote). The specific aims are: 1. Identification and evaluation (in vivo) of antigens protective against an infection with Leishmania sp.. 2. Elucidation of the effector mechanisms involved in a protective immune response against leishmaniasis. 3. Development of methods/strains for the axenic culture of Leishmania amastigotes. 4. Molecular biological, biochemical, and biological characterization of stage-specific antigens (promastigote and amastigote) of Leishmania. 5. Studies of the M-2 Gene Family. 6. Molecular cloning of stage-specific and protective antigens. 7. Development of a reliable specific serological diagnostic assay for visceral leishmaniasis. The aims of this proposal are essentially those of the original proposal; however, the methodology and approaches being taken have been expended based on the progress that has occurred during the past two years.

本提案的目的是继续和扩大