LEISHMANIA ANTIGENS-BIOCHEMICAL AND IMMUNOLOGIC STUDIES

利什曼原虫抗原-生化和免疫学研究

• 批准号: 3134811
• 负责人: Diane McMahon Pratt
• 金额: $ 26.13万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134811
• 关键词: Leishmania; Leishmania donovani; active immunization; affinity chromatography; communicable disease diagnosis; electron microscopy; enzyme linked immunosorbent assay; genetic manipulation; hamsters; high performance liquid chromatography; immunofluorescence technique; immunologic assay /test; immunological substance; ion exchange chromatography; laboratory mouse; laboratory rabbit; leishmaniasis; macrophage; molecular cloning; monoclonal antibody; parasitic disease chemotherapy; parasitic disease diagnosis; passive immunization; protein sequence; protozoal antigen; protozoal vaccine; tissue /cell culture; vaccines

The purpose of this proposal is to continue and extend the previous work supported by grant AI-23004, to delineate both the antigens and mechanisms involved in protective immunity for leishmaniasis. In addition, the proposal is to investigate biochemical differences responsible for the survival of the parasite in each of the life cycle stages (promastigote- amastigote). The specific aims are: 1. Identification and evaluation (in vivo) of antigens protective against an infection with Leishmania sp.. 2. Elucidation of the effector mechanisms involved in a protective immune response against leishmaniasis. 3. Development of methods/strains for the axenic culture of Leishmania amastigotes. 4. Molecular biological, biochemical, and biological characterization of stage-specific antigens (promastigote and amastigote) of Leishmania. 5. Studies of the M-2 Gene Family. 6. Molecular cloning of stage-specific and protective antigens. 7. Development of a reliable specific serological diagnostic assay for visceral leishmaniasis. The aims of this proposal are essentially those of the original proposal; however, the methodology and approaches being taken have been expended based on the progress that has occurred during the past two years.

这项建议的目的是继续和扩大 先前的工作得到了资助AI-23004的支持，以描述 保护性免疫的抗原和机制 黑热病 此外，该提案还将调查 生物化学差异负责的生存， 在每个生命周期阶段的寄生虫（前鞭毛体， 无鞭毛体）。 具体目标是： 1. 保护性抗原的鉴定和评价（体内） 对抗利什曼原虫感染 2. 阐明了参与一个 对利什曼病的保护性免疫反应。 3. 无菌培养方法/菌株的开发 利什曼原虫无鞭毛体 4. 分子生物学、生物化学和生物学 阶段特异性抗原（前鞭毛体和 无鞭毛体）。 5. M-2基因家族的研究 6. 阶段特异性和保护性抗原的分子克隆。 7. 一种可靠的特异性血清学诊断方法的建立 内脏利什曼病 这一建议的目的基本上是那些原来的 建议;然而，正在采取的方法和办法 根据已取得的进展， 在过去的两年里。