TRANSACTIVATION BY THE PSEUDORABLES VIRUS IEP PROTEIN

伪病毒 IEP 蛋白的反激活

• 批准号: 3130814
• 负责人: LAWRENCE T FELDMAN
• 金额: $ 15.08万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-01-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3130814
• 关键词: DNA binding protein; Escherichia coli; Herpesviridae disease; affinity chromatography; antiserum; binding proteins; gene expression; genetic enhancer element; genetic manipulation; genetic mapping; genetic promoter element; genetic regulation; genetic transcription; genotype; ligase; molecular weight; monoclonal antibody; nucleic acid sequence; suid alphaherpesvirus 1; tissue /cell culture; transcription factor; transposon /insertion element; virus genetics; virus protein

The major goals of this project are to examine the biochemical properties of the immediate-early protein of pseudorabies virus. This protein is responsible for the autoregulation of its own gene and the activation for the autoregulation of its own gene and the activation of early viral gene transcription in virus infected cells. The ability of this protein to interact with its own promoter region will be investigated. An interaction of the viral protein with a cellular DNA binding protein results in increased binding of the cellular protein to DNA. Using monoclonal antisera to the viral and the cellular protein, in vitro DNA binding assays, and in vitro transcription systems, the mechanisms of autoregulation and early viral gene activation will be determined. The results obtained from this study should be helpful in enhancing our understanding of the mechanisms of mammalian cell transcription. The identification of a possible cellular transcription factor and its role in viral transcription should eventually lead to an increased understanding of the interaction of viral and cellular proteins, and the role of this protein in cellular processes.

这个项目的主要目标是检查生物化学 伪狂犬病病毒即刻早期蛋白的性质。 这种蛋白质负责自身基因的自动调节。 以及激活自身基因的自动调节和 病毒感染细胞中早期病毒基因转录的激活。 这种蛋白质与其自身启动子相互作用的能力 将对地区进行调查。病毒蛋白的相互作用 与细胞DNA结合蛋白的结合导致结合增加 将细胞蛋白质转化为DNA。用抗鼠疫病毒的单抗血清 病毒和细胞蛋白，体外DNA结合分析，以及在 体外转录系统，自动调节机制和 病毒基因的早期激活将被确定。 从这项研究获得的结果应该有助于 加深我们对哺乳动物的机制的了解 细胞转录。一个可能的细胞的鉴定 转录因子及其在病毒转录中的作用 最终导致对相互作用的更多了解 病毒和细胞蛋白，以及这种蛋白在 细胞过程。

项目成果

Immediate-early protein of pseudorabies virus is not continuously required to reinitiate transcription of induced genes.

伪狂犬病病毒的立即早期蛋白并不持续需要重新启动诱导基因的转录。

• DOI: 10.1128/jvi.61.4.1258-1260.1987
• 发表时间: 1987
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Ahlers,SE;Feldman,LT
• 通讯作者: Feldman,LT

Binding of the pseudorabies virus immediate-early protein to single-stranded DNA.

伪狂犬病病毒立即早期蛋白与单链 DNA 的结合。

• DOI: 10.1128/jvi.61.6.1855-1860.1987
• 发表时间: 1987
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Chlan,CA;Coulter,C;Feldman,LT
• 通讯作者: Feldman,LT

Effects of a temperature-sensitive mutation in the immediate-early gene of pseudorabies virus on class II and class III gene transcription.

伪狂犬病病毒早期基因温度敏感突变对 II 类和 III 类基因转录的影响。

• DOI: 10.1128/jvi.61.4.1103-1107.1987
• 发表时间: 1987
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Ahlers,SE;Feldman,LT
• 通讯作者: Feldman,LT