TRANSACTIVATION BY THE PSEUDORABLES VIRUS IEP PROTEIN

该项目的主要目标是研究生物化学 伪狂犬病病毒即早蛋白的性质。 这种蛋白质负责自身基因的自动调节 以及自身基因的自动调节的激活 病毒感染细胞中早期病毒基因转录的激活。 这种蛋白质与自身启动子相互作用的能力 该地区将进行调查。 病毒蛋白的相互作用 与细胞DNA结合蛋白的结合导致 将细胞蛋白质转化为DNA 使用抗人血清的单克隆抗体 病毒和细胞蛋白，体外DNA结合试验，以及 体外转录系统，自动调节机制， 将确定早期病毒基因激活。 从这项研究中获得的结果应该有助于 提高我们对哺乳动物 细胞转录 一个可能的细胞的识别 转录因子及其在病毒转录中的作用， 最终会加深对 病毒和细胞蛋白质，以及这种蛋白质在 细胞过程

The major goals of this project are to examine the biochemical properties of the immediate-early protein of pseudorabies virus. This protein is responsible for the autoregulation of its own gene and the activation for the autoregulation of its own gene and the activation of early viral gene transcription in virus infected cells. The ability of this protein to interact with its own promoter region will be investigated. An interaction of the viral protein with a cellular DNA binding protein results in increased binding of the cellular protein to DNA. Using monoclonal antisera to the viral and the cellular protein, in vitro DNA binding assays, and in vitro transcription systems, the mechanisms of autoregulation and early viral gene activation will be determined. The results obtained from this study should be helpful in enhancing our understanding of the mechanisms of mammalian cell transcription. The identification of a possible cellular transcription factor and its role in viral transcription should eventually lead to an increased understanding of the interaction of viral and cellular proteins, and the role of this protein in cellular processes.