LEISHMANIA ANTIGENS-BIOCHEMICAL AND IMMUNOLOGIC STUDIES

利什曼原虫抗原-生化和免疫学研究

• 批准号: 3134803
• 负责人: Diane McMahon Pratt
• 金额: $ 16.06万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1988-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134803
• 关键词: Leishmania; Leishmania donovani; active immunization; communicable disease diagnosis; electron microscopy; enzyme linked immunosorbent assay; genetic manipulation; high performance liquid chromatography; immunofluorescence technique; immunologic assay /test; immunological substance; leishmaniasis; macrophage; molecular cloning; monoclonal antibody; parasitic disease diagnosis; passive immunization; protozoal antigen; protozoal vaccine; tissue /cell culture

This proposal is primarily focused on delineating the antigens relevant for protective immunity against leishmaniasis and clinical diagnosis of the disease (i.e., Kala-azar) as well as developing a further understanding of the biology of the parasite (i.e., its survival within its insect and mammalian hosts). The projects will involve the disciplines of biology, immunology, molecular biology and biochemistry. Monoclonal antibodies and molecular biology techniques will be used to identify and isolate molecules (stage specific and protective) of Leishmania. The specific aims of the proposal are: 1. Development of a reliable, specific serological diagnostic assay for visceral leishmaniasis. 2. Identification of antigens protective against infection with Leishmania donovani. 3. Further production of amastigote specific monoclonal antibodies to the amastigote stage. 4. Biochemical, biological and molecular biological characterization of stage-specific antigens (promastigote and amastigote) of Leishmania. 5. Evaluation of stage-specific antigens effectiveness of producing protective immunity in in vivo model systems. 6. Elucidation of mechanisms involved in protective immunity produced to both stage specific and cross-reactive antigens. 7. Analysis of Leishmania antigens on infected macrophages.

该建议主要集中在描绘与以下相关的抗原： 利什曼病保护性免疫与临床诊断 疾病（即，黑热病），以及进一步了解 寄生虫的生物学（即，它在昆虫体内的生存， 哺乳动物宿主）。 这些项目将涉及生物学， 免疫学、分子生物学和生物化学。 单克隆抗体和 分子生物学技术将用于识别和分离分子 （阶段特异性和保护性）利什曼原虫。 该提案的具体目标是： 1.建立一种可靠、特异的血清学诊断方法， 内脏利什曼病 2.抗利什曼原虫感染保护性抗原的鉴定 donovani。 3.进一步产生针对无鞭毛体的特异性单克隆抗体， 无鞭毛期 4.生物化学、生物学和分子生物学表征 利什曼原虫阶段特异性抗原（前鞭毛体和无鞭毛体）。 5.阶段特异性抗原生产有效性的评价 体内模型系统中的保护性免疫。 6.阐明产生保护性免疫的机制， 阶段特异性和交叉反应性抗原。 7.感染巨噬细胞上利什曼原虫抗原的分析。