CELL SURFACE PROTEIN OF BACILLUS SPHAERICUS

球形芽孢杆菌的细菌沉积了一种高分子量 细胞外表面的蛋白质。在某些菌株中，这种蛋白质 显示规则的亚基阵列(RS或T层)，但为其他菌株 蛋白质层为非规则结构(NRS层)。后者 对于所有对蚊子有致病作用的菌株，情况都是如此 幼虫。这些致病菌株中的一些会产生一种非常有效的 针对蚊子幼虫的特定毒素正在考虑开发 作为杀幼虫剂。它们可能会提供一种有用的新工具来控制 病媒传播的疾病。这个项目的总体目标是检查一些 大分子NRS蛋白在细胞上的性质 一些蚊虫致病菌株的表面球状芽孢杆菌。这 将通过比较蛋白质的分子重量来完成 等电聚焦的等电点，以及 通过肽图谱分析它们的组成。NRS蛋白也将是 与其他选定菌株的RS蛋白进行比较。美国政府的角色 蛋白质作为噬菌体的吸附部位将被研究。一个 要检验的假设是，NRS蛋白是吸附的 噬菌体分型方案中使用的10个噬菌体的位点，以及 表面蛋白将在生物化学和/或免疫学上 可在噬菌体群中区分的。坚持的特殊性是 存在于NRS蛋白和其下层的肽聚糖球囊之间 也将被确定。该项目是长期计划的一部分 更好地理解这些结构与功能的关系 细菌。表面蛋白可能在确定 这些细菌在昆虫幼虫体内的生存和/或生长 他们。这些蛋白质在实际应用方面也可能很重要。 将细菌制成杀幼虫剂。

Bacteria of the species Bacillus sphaericus deposit a high molecular weight protein on the outer surface of the cell. In some strains this protein displays a regular array of subunits (RS or T layer), but in other strains the protein layer is non-regularly structured (NRS layer). The latter situation is true for all of the strains which are pathogenic for mosquito larvae. Some of these pathogenic strains which produce a potent and very specific toxin against mosquito larvae are being considered for development as larvicides. They may provide a useful new tool for the control of vector borne diseases. The overall goal of this project is to examine some of the properties of the high molecular weight NRS protein on the cell surface of a number of mosquito pathogenic strains of B. sphaericus. This will be done by comparing melecular weights of the proteins by electrophoresis, their isoelectric points by isoelectric focusing, and their composition by peptide mapping. The NRS proteins will also be compared to RS proteins from other selected strains. The role of the proteins as bacteriophage adsorption sites will be investigated. A hypothesis is to be tested is that the NRS proteins are the adsorption sites for 10 bacteriophages used in a phage typing scheme and that the surface proteins will be biochemically and or immunologically distinguishable among the phage groups. The specificity of adherence that exists between the NRS protein and the underlying peptidoglycan sacculus will also be determined. This project is part of the long range plan to obtain a better understanding of structure-function relationships in these bacteria. The surface proteins may be important in determining the survival and/or growth of these bacteria in insect larvae which consume them. The proteins may also be important in practical aspects of formulating the bacteria into larvicides.