IDIOTYPE NETWORKS IN TRANSFUSION-AIDS

输血辅助中的独特型网络

• 批准号: 3135615
• 负责人: GORDON R DREESMAN
• 金额: $ 16.42万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3135615
• 关键词: AIDS; affinity chromatography; antiidiotype antibody; blood donor; blood transfusion; communicable disease control; communicable disease transmission; enzyme linked immunosorbent assay; gene expression; human T cell lymphotropic virus type 1; human tissue; immunoelectron microscopy; immunological substance; leukocyte activation /transformation; serology /serodiagnosis; synthetic peptide; viral vaccines; virus antigen

Transfusion-associated AIDS has become a major health problem as clearly shown by serologic evidence in the general public and by transmission of the disease to chimpanzees with plasma from AIDS patients. The characterization of idiotypic determinants and the generation of anti-idiotypic reagents have provided powerful tools with which to analyze and modulate a given immune response. The idiotype network developed during the course of an immune response provides conceptual insight into the host response to a given antigen. It is proposed to analyze the idiotype network initiated by a host in response to a human T-cell lymphotropic virus type III (HTLV-III) infection or immunization with HTLV-III. The idiotypes that are expressed on human, chimpanzee and mouse antibodies to the envelope glycoproteins, gp 120 and gp 41, of HTLV-III will be examined. The expression of these idiotypic determinants on antibodies to gp 120 and gp 41 produced in naturally infected and immunized individuals will be analyzed. The identification of a major common idiotype(s) in human sera positive for anti-gp 120 and anti-gp 41 will be used to detect potential variable region antibody gene differences among AIDS diagnosed individuals and in blood donors containing antibody but having no disease. The epitopes associated with gp 120 and gp 41 and with synthetic gp 120 and gp 41 peptides will be identified. In addition, we will examine the use of anti-idiotypic antibodies for an effective safe vaccine. The results of these experiments will give insight into whether idiotype networks, expression of particular idiotypes or both are important in the host's response to HTLV-III. These anti-idiotype reagents may also identify serologic markers as indicators of infectivity.

输血相关的艾滋病已成为一个主要的健康问题， 一般公众的血清学证据和 用艾滋病患者的血浆给黑猩猩注射艾滋病病毒。 的 独特型决定因素的表征和 抗独特型试剂提供了强大的工具， 并调节特定的免疫反应。 独特型网络发展了 在免疫反应过程中， 宿主对给定抗原的反应。 建议分析 由宿主响应人T细胞启动的独特型网络 嗜淋巴细胞病毒III型（HTLV-III）感染或免疫 HTLV-III。 在人、黑猩猩和小鼠上表达的独特型 抗HTLV-III包膜糖蛋白gp 120和gp 41的抗体 将被审查。 这些独特型决定因素在细胞上的表达 自然感染和免疫后产生的针对gp 120和gp 41的抗体 将对个人进行分析。 确定一个主要共同点 抗gp120和抗gp41阳性人血清中的独特型将被 用于检测潜在的可变区抗体基因差异， 艾滋病确诊者和献血者中含有抗体， 没有疾病。 与gp120和gp41相关的表位以及与 将鉴定合成的GP 120和GP 41肽。 另外我们 将检查抗独特型抗体的使用， 疫苗 这些实验的结果将使我们深入了解， 独特型网络、特定独特型的表达或两者都很重要 对HTLV-III病毒的反应 这些抗独特型试剂还可以 确定血清学标志物作为传染性指标。