IDIOTYPE NETWORKS IN TRANSFUSION-AIDS

与输血相关的艾滋病显然已经成为一个主要的健康问题 在普通公众中的血清学证据和通过传播 这种疾病给黑猩猩带来了艾滋病患者的血浆。这个 独特型决定因素的表征和独特型决定因素的产生 反独特型试剂为分析提供了强大的工具 并调节给定的免疫反应。独特型网络发展起来 在免疫反应过程中提供了概念上的洞察 宿主对给定抗原的反应。建议分析一下 由宿主启动的响应人类T细胞的独特型网络 嗜淋巴病毒III(HTLV-III)感染或免疫 HTLV-III。在人、黑猩猩和小鼠身上表达的独特型 抗HTLV-III囊膜糖蛋白gP 120和gP 41的抗体 将会被检查。这些独特型决定因素在人类基因组中的表达 自然感染和免疫后产生的抗GP 120和GP 41抗体 将对个人进行分析。确定一个主要的共同之处 在抗GP120和抗GP41阳性的人血清中独特型(S) 用来检测人群中潜在可变区抗体基因的差异 艾滋病确诊个体和含有抗体的献血者，但 没有病的没有病的与GP 120和GP 41相关的表位以及与 人工合成的GP 120和GP 41多肽将被鉴定。此外，我们 将检查使用抗独特型抗体是否有效安全 疫苗。这些实验的结果将让我们深入了解 独特型网络、特定独特型的表达或两者兼而有之 在宿主对HTLV-III的反应中。这些抗独特型试剂也可能 确定血清标志物作为传染性的指示物。

Transfusion-associated AIDS has become a major health problem as clearly shown by serologic evidence in the general public and by transmission of the disease to chimpanzees with plasma from AIDS patients. The characterization of idiotypic determinants and the generation of anti-idiotypic reagents have provided powerful tools with which to analyze and modulate a given immune response. The idiotype network developed during the course of an immune response provides conceptual insight into the host response to a given antigen. It is proposed to analyze the idiotype network initiated by a host in response to a human T-cell lymphotropic virus type III (HTLV-III) infection or immunization with HTLV-III. The idiotypes that are expressed on human, chimpanzee and mouse antibodies to the envelope glycoproteins, gp 120 and gp 41, of HTLV-III will be examined. The expression of these idiotypic determinants on antibodies to gp 120 and gp 41 produced in naturally infected and immunized individuals will be analyzed. The identification of a major common idiotype(s) in human sera positive for anti-gp 120 and anti-gp 41 will be used to detect potential variable region antibody gene differences among AIDS diagnosed individuals and in blood donors containing antibody but having no disease. The epitopes associated with gp 120 and gp 41 and with synthetic gp 120 and gp 41 peptides will be identified. In addition, we will examine the use of anti-idiotypic antibodies for an effective safe vaccine. The results of these experiments will give insight into whether idiotype networks, expression of particular idiotypes or both are important in the host's response to HTLV-III. These anti-idiotype reagents may also identify serologic markers as indicators of infectivity.