ASSAY METHODS TO DETECT ANTIGENIC MARKERS FOR AIDS

本项目的主要目标是确定与S相关的唯一抗原 携带者状态为获得性免疫缺陷综合征(AIDS)。在……里面 鉴于此，我们建议开发灵敏和特异的免疫分析方法来 检测这些独特的抗原(S)。这些免疫分析将被迅速用于 确定无症状的艾滋病携带者。第一个目标 是研究一组来自几个患者群体的人类血清 代表被诊断为患有疾病、有患病风险的个人 推测未知的抗体的发展和各自的控制 可能存在于所获得的材料或组织中的可传播剂 来自艾滋/卡波西的肉瘤复合体。此外，多克隆和 针对推定病原体(S)不明抗原的单抗 将被生产并用于筛查被诊断为 艾滋病和卡波西肉瘤患者的组织。第二，我们将 用纯化的血浆组分接种非人类灵长类动物 艾滋病患者。这些动物的免疫反应将被跟踪 用于：1)开发与艾滋病相关的药物的抗体，以及2) 由急性疾病或携带者疾病引起的独特抗原的存在 在受感染的非人类灵长类动物中诱导的状态。这些试剂是 产生或鉴定的物质将用于快速和灵敏的固体 时相放射免疫分析(SPRIA)与生物素-亲和素扩增酶联 免疫吸附试验(A-EL ISA)用于艾滋病标志物的特异性检测。

The major goal of this project is to identify unique antigen(s) associated with the carrier state of Acquired Immune Deficiency Syndrome (AIDS). In this light, we propose to develop sensitive and specific immunoassays to detect these unique antigen(s). These immunoassays will be used to rapidly identify individuals who are asymptomatic carriers of AIDS. The first aim is to study a panel of human sera derived from several patient populations that represent individuals diagnosed with disease, at risk for disease development, and respective controls for antibody to putative unknown transmissible agents which may be present in material or tissue obtained from AIDS/Kaposi's sarcoma complex. In addition, both polyclonal and monoclonal antibody to an unidentified antigen of the putative agent(s) will be produced and used to screen blood from individuals diagnosed with AIDS and tissue from patients with Kaposi's sarcoma. Second, we will inoculate nonhuman primates with purified plasma fractions derived from patients with AIDS. The immune response of these animals will be followed for: 1) the development of antibody to agents associated with AIDS, and 2) the presence of unique antigens resulting from acute or carrier disease states induced in infected nonhuman primates. The reagents that were generated or identified will be used to develop rapid and sensitive solid phase radioimmunoassay (SPRIA) and biotin-avidin amplified enzyme linked immunosorbent assays (A-ELISA) for specific detection of markers for AIDS.