ASSAY METHODS TO DETECT ANTIGENIC MARKERS FOR AIDS

检测艾滋病抗原标记的测定方法

• 批准号: 3343854
• 负责人: GORDON R DREESMAN
• 金额: $ 16.31万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1987-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3343854
• 关键词: AIDS; Betaherpesvirinae; Kaposi's sarcoma; antiviral antibody; communicable disease diagnosis; disease /disorder model; disease carrier state; enzyme linked immunosorbent assay; human T cell lymphotropic virus type 1; human subject; hybridomas; immunofluorescence technique; immunopathology diagnosis; monoclonal antibody; radioimmunoassay; serology /serodiagnosis; virus antigen

The major goal of this project is to identify unique antigen(s) associated with the carrier state of Acquired Immune Deficiency Syndrome (AIDS). In this light, we propose to develop sensitive and specific immunoassays to detect these unique antigen(s). These immunoassays will be used to rapidly identify individuals who are asymptomatic carriers of AIDS. The first aim is to study a panel of human sera derived from several patient populations that represent individuals diagnosed with disease, at risk for disease development, and respective controls for antibody to putative unknown transmissible agents which may be present in material or tissue obtained from AIDS/Kaposi's sarcoma complex. In addition, both polyclonal and monoclonal antibody to an unidentified antigen of the putative agent(s) will be produced and used to screen blood from individuals diagnosed with AIDS and tissue from patients with Kaposi's sarcoma. Second, we will inoculate nonhuman primates with purified plasma fractions derived from patients with AIDS. The immune response of these animals will be followed for: 1) the development of antibody to agents associated with AIDS, and 2) the presence of unique antigens resulting from acute or carrier disease states induced in infected nonhuman primates. The reagents that were generated or identified will be used to develop rapid and sensitive solid phase radioimmunoassay (SPRIA) and biotin-avidin amplified enzyme linked immunosorbent assays (A-ELISA) for specific detection of markers for AIDS.

该项目的主要目标是鉴定与免疫相关的独特抗原。 患有获得性免疫缺陷综合症（艾滋病）的携带者状态。 在 鉴于此，我们建议开发灵敏和特异的免疫测定法， 检测这些独特的抗原。 这些免疫测定将用于快速 确定无症状的艾滋病携带者。 第一个目标 是研究一组来自几个病人群体的人类血清 代表被诊断患有疾病的个体， 开发，以及针对推定未知抗体的相应对照 可能存在于获得的材料或组织中的传染性因子 艾滋病/卡波西氏肉瘤综合征 此外，多克隆和 针对推定病原体的未鉴别抗原的单克隆抗体 将被生产并用于筛查被诊断患有 艾滋病和卡波西肉瘤患者的组织。 二是 用来自以下动物的纯化血浆级分对非人灵长类动物进行免疫 艾滋病患者。 这些动物的免疫反应将被跟踪 用于：1）产生与艾滋病相关的抗体，以及2） 急性或携带者疾病导致的独特抗原的存在 在受感染的非人灵长类动物中诱导的状态。 所用的试剂 产生或确定的将用于开发快速和敏感的固体 相放射免疫分析法（SPRIA）和生物素-亲和素放大酶联免疫法 免疫吸附测定（A-ELISA）用于特异性检测AIDS标志物。