TN3 TRANSPOSITION IMMUNITY: A NEW GENETIC PHENOMENON

TN3 转座免疫：一种新的遗传现象

• 批准号: 3130857
• 负责人: FRED L HEFFRON
• 金额: $ 11.65万
• 依托单位: SCRIPPS CLINIC AND RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-01 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3130857
• 关键词: Escherichia coli; R factors; bacterial genetics; beta lactamase; chromosome deletion; chromosome inversion; chromosome translocation; gene expression; gene redundancy; genetic manipulation; molecular cloning; nuclease; nucleic acid chemical synthesis; nucleic acid sequence; nucleic acid structure

Transposition immunity is a relatively unstudied genetic phenomenon in bacteria. Tn3 transposition immunity is a cis acting mechanism which limits Tn3 insertion into a plasmid. We have shown that Tn3's terminal repeat can confer immunity to a plasmid. However, we also find that the transposition immunity phenotype can be affected by host sequences. During this granting period we will pursue a genetic approach, using techniques of in vitro and in vivo mutagenesis, to dissect the immunity sequence as well as host and plasmid sequences which also affect immunity. We will determine whether other transposons related to Tn3 show immunity and if so determine whether they show cross immunity to each other. We will determine to how large a DNA molecule Tn3 can confer immunity and whether there are other parameters which also affect immunity. For example, some naturally occurring plasmids are immune to Tn3 though they do not confer penicillin resistance. Through this genetic approach, as well as additional experiments, we will distinguish between the various models for how transposition immunity works.

转座免疫是一种相对未研究的遗传现象， 细菌Tn3转座免疫是一种顺式作用机制， Tn3插入质粒。 我们已经证明Tn3的末端重复序列可以 赋予质粒免疫力。 然而，我们也发现， 免疫表型可受宿主序列影响。 在此授予 在此期间，我们将采用基因方法，使用体外技术， 体内诱变，以剖析免疫序列以及宿主， 也影响免疫力的质粒序列。 我们将决定 与Tn3相关的其他转座子显示免疫性，如果是，则确定是否 它们对彼此表现出交叉免疫 我们将确定一个 DNA分子Tn3可以赋予免疫力以及是否还有其他参数 这也影响免疫力。 例如，一些天然存在的质粒 对Tn3免疫，但不赋予青霉素抗性。 通过 这种遗传方法，以及额外的实验，我们将 区分换位免疫如何工作的各种模型。

项目成果

Shuttle mutagenesis: bacterial transposons for genetic manipulations in yeast.

穿梭诱变：用于酵母遗传操作的细菌转座子。

• DOI: 10.1016/0076-6879(91)94025-8
• 发表时间: 1991
• 期刊: Methods in enzymology
• 作者: Hoekstra,MF;Seifert,HS;Nickoloff,J;Heffron,F
• 通讯作者: Heffron,F

Analysis of Tn3 sequences required for transposition and immunity.

转座和免疫所需的 Tn3 序列分析。

• DOI: 10.1016/0378-1119(86)90263-5
• 发表时间: 1986
• 期刊: Gene
• 影响因子: 3.5
• 作者: Huang,CJ;Heffron,F;Twu,JS;Schloemer,RH;Lee,CH
• 通讯作者: Lee,CH

Transposon Tn5 mutagenesis of Brucella abortus.

流产布鲁氏菌的转座子 Tn5 诱变。

• DOI: 10.1128/iai.55.11.2774-2776.1987
• 发表时间: 1987
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Smith,LD;Heffron,F
• 通讯作者: Heffron,F