MEASLES VIRUS GENE FUNCTIONS BY DNA MANIPULATION

通过 DNA 操作研究麻疹病毒基因功能

• 批准号: 3136078
• 负责人: TIMOTHY C. WONG
• 金额: $ 11.86万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3136078
• 关键词: Cercopithecidae; RNA virus; complementary DNA; density gradient ultracentrifugation; endonuclease; gel electrophoresis; gene complementation; gene expression; gene mutation; genetic library; genetic manipulation; genetic promoter element; genetic transcription; genetic translation; immunoprecipitation; membrane proteins; messenger RNA; molecular cloning; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; subacute sclerosing panencephalitis; tissue /cell culture; virus protein; virus replication

The proposed project is intended to spear-head a comprehensive research program to develop new practical strategies for analyzing the gene functions of RNA viruses by genetic manipulation of the cloned genes. In this project, we aimed at converting the entire spectrum of the gene expression products of measles virus from mRNAs into functional and expressible cDNA clones. By gene transfer, specific genes of the measles virus will be expressed in environments amenable to detail biochemical and functional analyses. The effects of over-expression and under-expression of specific viral genes on the replication of measles virus will be critically examined to gain insight into the biological roles of the viral genes. The materials and knowledge thus obtained will be used to fully define the putative detect(s) in the genomes of several strains of subacute sclerosing panencephalitis (SSPE) viruses. Specific SSPE viral genes will be cloned and the putative defect(s) will be characterized at the structural and functional levels. Novel approaches will be developed to generate specific recombinant RNA viruses via cloned gene-mediated complementation ad rescue experiments.

拟议中的项目旨在带头建立一个全面的 研究计划，以制定新的实用战略 从基因水平分析RNA病毒的基因功能 对克隆基因的操纵。在这个项目中，我们的目标是 将基因表达产物的整个光谱转化为 将麻疹病毒从mRNAs转化为有功能和可表达的 CDNA克隆。通过基因转移，麻疹的特异性基因 病毒将在服从细节的环境中表达 生化和功能分析。的影响 鸡传染性支气管炎病毒特异性基因的高表达和低表达 将对麻疹病毒的复制进行严格检查，以 深入了解病毒基因的生物学作用。这个 这样获得的材料和知识将被充分利用 确定在几个菌株基因组中推测的检测(S) 亚急性硬化性全脑炎(SSPE)病毒。 将克隆特定的SSPE病毒基因，并推测 缺陷(S)将在结构上和结构上进行表征 功能层次。将开发新的方法来 通过克隆产生特异的重组RNA病毒 基因介导的互补和拯救实验。

项目成果

Generalized and localized biased hypermutation affecting the matrix gene of a measles virus strain that causes subacute sclerosing panencephalitis.

影响导致亚急性硬化性全脑炎的麻疹病毒株基质基因的全身性和局部性偏向超突变。

• DOI: 10.1128/jvi.63.12.5464-5468.1989
• 发表时间: 1989
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Wong,TC;Ayata,M;Hirano,A;Yoshikawa,Y;Tsuruoka,H;Yamanouchi,K
• 通讯作者: Yamanouchi,K

Functional interaction between transcriptional elements in the long terminal repeat of reticuloendotheliosis virus: cooperative DNA binding of promoter- and enhancer-specific factors.

网状内皮增生病毒长末端重复中转录元件之间的功能相互作用：启动子和增强子特异性因子的协同DNA结合。

• DOI: 10.1128/mcb.8.12.5232-5244.1988
• 发表时间: 1988
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Hirano,A;Wong,T
• 通讯作者: Wong,T