MEASLES VIRUS GENE FUNCTIONS BY DNA MANIPULATION

通过 DNA 操作研究麻疹病毒基因功能

• 批准号: 3136074
• 负责人: TIMOTHY C. WONG
• 金额: $ 11.77万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3136074
• 关键词: RNA virus; complementary DNA; density gradient ultracentrifugation; endonuclease; gel electrophoresis; gene complementation; gene expression; gene mutation; genetic library; genetic manipulation; genetic transcription; genetic translation; immunoprecipitation; messenger RNA; molecular cloning; nucleic acid sequence; subacute sclerosing panencephalitis; tissue /cell culture; virus replication

The proposed project is intended to spear-head a comprehensive research program to develop new practical strategies for analyzing the gene functions of RNA viruses by genetic manipulation of the cloned genes. In this project, we aimed at converting the entire spectrum of the gene expression products of measles virus from mRNAs into functional and expressible cDNA clones. By gene transfer, specific genes of the measles virus will be expressed in environments amenable to detail biochemical and functional analyses. The effects of over-expression and under-expression of specific viral genes on the replication of measles virus will be critically examined to gain insight into the biological roles of the viral genes. The materials and knowledge thus obtained will be used to fully define the putative detect(s) in the genomes of several strains of subacute sclerosing panencephalitis (SSPE) viruses. Specific SSPE viral genes will be cloned and the putative defect(s) will be characterized at the structural and functional levels. Novel approaches will be developed to generate specific recombinant RNA viruses via cloned gene-mediated complementation ad rescue experiments.

拟议的项目旨在带头进行全面的 研究计划，以制定新的实用战略， 用遗传学方法分析RNA病毒的基因功能 操纵克隆基因。 在这个项目中，我们的目标是 将整个基因表达产物谱 麻疹病毒从mRNA到功能和表达 cDNA克隆。 通过基因转移，麻疹的特定基因 病毒将在适合于细节的环境中表达 生化和功能分析。 的影响 特定病毒基因的过表达和表达不足 麻疹病毒的复制将受到严格检查， 深入了解病毒基因的生物学作用。 的 这样获得的材料和知识将用于充分利用 确定几种菌株基因组中的推定检出 亚急性硬化性全脑炎（SSPE）病毒。 将克隆特定的SSPE病毒基因，并将推定的SSPE病毒基因克隆到细胞中。 缺陷将在结构和 功能层次。 将开发新的方法， 通过克隆产生特异性重组RNA病毒 基因介导的互补和拯救实验。