STRUCTURE & REGULATION OF HEPATOCYTE STIMULATING FACTOR

任何病因的急性炎症都会引发一系列公认的 有机体内的分子和细胞变化。包括在此 反应是一种改变，合成和分泌一些 来自肝脏的血浆蛋白。我们最近证明了 单核细胞和巨噬细胞被激活时，会分泌一种蛋白质因子，这种蛋白质因子 显著影响几种肝细胞源性血浆的合成 蛋白质。这项提议的长期目标是(A)隔离和 对这种常规蛋白质进行化学表征，(B)了解 触发单核细胞中调节因子的合成和(C) 确定调节蛋白如何刺激肝脏产生 某些血浆蛋白。其中一些研究将会进行。 在单层培养系统中使用肝细胞和单核细胞。我们有 已经确定培养中的原代肝细胞对 单核细胞因子以类似于体内反应的方式。此外， 我们已经开发了一种特定的定量生物测定方法，将用于 提纯肝刺激因子。这些研究的结果将 提供有关一种重要物质的化学和作用方式的新信息 与急性炎症密切相关的调节蛋白 反应。由于急性炎症反应的发生是由于 创伤和疾病状态的数量，对化学和 引起反应的因素(S)的作用对 了解炎症的分子机制。

Acute inflammation of any etiology initiates a well recognized sequence of molecular and cellular changes within an organism. Included in this response is an alteration the synthesis and secretion of a number of hepatically derived plasma proteins. We have recently demonstrated that monocytes and macrophages, when activated, secrete a protein factor which significantly effects the synthesis of several hepatocyte derived plasma proteins. The long range objective of this proposal is (a) to isolate and chemically characterize this regular protein, (b) to understand what triggers the synthesis of the regulator in the monocyte and (c) to determine how the regulator protein stimulates the hepatic production of certain plasma proteins. A number of these studies will be carried out using hepatocytes and monocytes in monolayer culture systems. We have already established that primary hepatocytes in culture respond to the monocytic factor in a manner analogous to in vivo response. Furthermore, we have developed a specific quantitative bioassay which will be used to purify the hepatic stimulating factor. Results from these studies will provide new information on the chemistry and mode of action of an important regulator protein that is intimately involved in the acute inflammatory reaction. Since an acute inflammatory reaction occurs as a result of a number of trauma and disease states, knowledge of the chemistry and function of the factor(s) involved in causing the response is crucial to understanding the molecular mechanism of inflammation.