MELT TRANSITION AND STRUCTURE OF MEIBOMIAN LIPIDS

睑板腺脂质的熔化转变和结构

• 批准号: 8362358
• 负责人: GERALD M FULLER
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362358
• 关键词: Air; Body Temperature; Cholesterol Esters; Disease; Esters; Eye; Eye diseases; Fatty Acids; Film; Funding; Goals; Grant; Individual; Influentials; Link; Lipid A; Lipids; Mechanics; National Center for Research Resources; Patients; Principal Investigator; Property; Radiation; Research; Research Infrastructure; Resources; Source; Structure; Surface; Temperature; United States National Institutes of Health; Waxes; chemical property; cost; eye dryness; interest; melting; monolayer; physical property; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Meibomian lipids are the major component of the outermost layer of the tear film, the film that protects the surface of the eye. They are composed of a wide variety of lipid species, the majority of which are long chain wax esters, cholesterol esters and fatty acids. Because it forms the interface with air, the physical and chemical properties of the lipid layer are believed to be influential in tear film stability. We are interested in exploring the structure of Meibomian lipids from individuals with stable tear films and patients with dry eye disease (unstable tear films). Studies of Meibomian lipid monolayers have shown the mechanical properties of these lipids to be quite responsive to temperature. We would like to link these results to changes in the crystalline structure from 25¿C through the melting transition, which will indicate the melt temperature of the lipids. In some forms of dry eye disease the composition of these lipids is different than asymptomatic individuals. If the melt temperature changes significantly compared to body temperature, the ability of the lipids to spread and the mechanical properties of the lipid layer may be modified in such a way that the tear film becomes unstable. The goal of this study is to understand the inherent crystalline structure of Meibum from ?normal? and dry eye subjects as a function of temperature using small angle x-ray scattering. We hope that such studies will contribute to an understanding of which properties promote stability in the tear film, how they change with disease, and how they can be restored for individuals suffering from dry eye.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 睑板脂是泪膜最外层的主要成分，泪膜是保护眼睛表面的膜。它们由多种脂质物质组成，其中大多数是长链蜡酯、胆固醇酯和脂肪酸。由于脂质层形成与空气的界面，因此脂质层的物理和化学性质被认为对泪膜稳定性有影响。我们有兴趣探索具有稳定泪膜的个体和患有干眼病（不稳定泪膜）的患者的睑板膜脂质的结构。对睑板腺脂质单层的研究表明，这些脂质的机械性质对温度非常敏感。我们希望将这些结果与晶体结构从25 ℃到熔融转变的变化联系起来，这将表明脂质的熔融温度。在某些形式的干眼病中，这些脂质的组成与无症状个体不同。如果熔化温度与体温相比显著变化，则脂质扩散的能力和脂质层的机械性质可能以泪膜变得不稳定的方式改变。本研究的目的是了解固有的晶体结构的Meibum从？正常吗？和干眼症受试者作为温度的函数。我们希望这样的研究将有助于了解哪些特性促进泪膜的稳定性，它们如何随疾病而变化，以及如何为患有干眼症的个体恢复。