MELT TRANSITION AND STRUCTURE OF MEIBOMIAN LIPIDS

睑板腺脂质的熔化转变和结构

• 批准号: 8170363
• 负责人: GERALD M FULLER
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170363
• 关键词: Air; Body Temperature; Cholesterol Esters; Computer Retrieval of Information on Scientific Projects Database; Disease; Esters; Eye; Eye diseases; Fatty Acids; Film; Funding; Goals; Grant; Individual; Influentials; Institution; Link; Lipids; Mechanics; Patients; Property; Research; Research Personnel; Resources; Source; Structure; Surface; Temperature; United States National Institutes of Health; Waxes; chemical property; eye dryness; interest; melting; monolayer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Meibomian lipids are the major component of the outermost layer of the tear film, the film that protects the surface of the eye. They are composed of a wide variety of lipid species, the majority of which are long chain wax esters, cholesterol esters and fatty acids. Because it forms the interface with air, the physical and chemical properties of the lipid layer are believed to be influential in tear film stability. We are interested in exploring the structure of Meibomian lipids from individuals with stable tear films and patients with dry eye disease (unstable tear films). Studies of Meibomian lipid monolayers have shown the mechanical properties of these lipids to be quite responsive to temperature. We would like to link these results to changes in the crystalline structure from 25¿C through the melting transition, which will indicate the melt temperature of the lipids. In some forms of dry eye disease the composition of these lipids is different than asymptomatic individuals. If the melt temperature changes significantly compared to body temperature, the ability of the lipids to spread and the mechanical properties of the lipid layer may be modified in such a way that the tear film becomes unstable. The goal of this study is to understand the inherent crystalline structure of Meibum from ?normal? and dry eye subjects as a function of temperature using small angle x-ray scattering. We hope that such studies will contribute to an understanding of which properties promote stability in the tear film, how they change with disease, and how they can be restored for individuals suffering from dry eye.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 睑板腺脂质是泪膜最外层的主要成分，泪膜是保护眼睛表面的薄膜。它们由多种脂质组成，其中大部分是长链蜡酯、胆固醇酯和脂肪酸。因为它与空气形成界面，所以脂质层的物理和化学性质被认为对泪膜稳定性有影响。我们有兴趣探索具有稳定泪膜的个体和患有干眼病（不稳定泪膜）的患者的睑板腺脂质的结构。对睑板腺单层脂质的研究表明，这些脂质的机械特性对温度非常敏感。我们希望将这些结果与从 25°C 到熔化转变期间晶体结构的变化联系起来，这将表明脂质的熔化温度。在某些形式的干眼病中，这些脂质的成分与无症状个体不同。如果融化温度与体温相比发生显着变化，则脂质的扩散能力和脂质层的机械特性可能会发生改变，从而导致泪膜变得不稳定。这项研究的目的是从“正常”状态了解睑脂的固有晶体结构。使用小角度 X 射线散射将干眼受试者作为温度的函数。我们希望此类研究将有助于了解哪些特性可以促进泪膜的稳定性，它们如何随疾病而变化，以及如何为患有干眼症的人恢复泪膜的稳定性。