MONOCLONAL ANTIBODIES TO HUMAN EPIDERMIS

人类表皮单克隆抗体

• 批准号: 3155932
• 负责人: LOWELL Alan GOLDSMITH
• 金额: $ 15.05万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3155932
• 关键词: aging; basal cell carcinoma; cell differentiation; cell fusion; cell growth regulation; cell membrane; cell population study; cell sorting; cellular immunity; chromosome movement; clone cells; congenital ichthyosis; cytogenetics; drug resistance; electrofocusing; electron microscopy; embryo /fetus; epidermolysis bullosa; fibroblasts; genetic mapping; human papillomavirus; human tissue; hybrid cells; hybridomas; immunofluorescence technique; immunogenetics; immunoglobulins; karyotype; keratoacanthoma; keratosis follicularis; lung neoplasms; lymphatic tissue; lymphoma; major histocompatibility complex; membrane activity; membrane structure; molecular biology; monoclonal antibody; multiple myeloma; neoplasm /cancer genetics; neoplasm /cancer invasiveness; neoplasm /cancer pharmacology; neoplastic cell; orphan disease /drug; pathologic keratinization; pemphigus; psoriasis; radioimmunoassay; skin; skin neoplasms; spleen; surface antigens; tumor antigens

Monoclonal antibodies to epidermal and basement membrane zone antigens have been formed and new antibodies will be formed by fusing spleen cells from immunized mice with the mouse myeloma cell line P3/NS1. Mice immunized with a human cervical carcinoma cell line (ME-180) have formed an antibody to human anchoring fibrils. The antigen in human skin and ME-180 will be completely characterized with its molecular weight, isoelectric point, peptide and cyanogen bromide map and amino acid composition. The cell of origin of the antigen in skin will be determined by biosynthetic studies and dermal/epidermal recombinations on the nude mouse. The formation of the anchoring fibril and its antigen will be sequentially followed during wound healing and during epidermal cell attachment to collagen-coated tissue culture dishes. Gene mapping of the anchoring fibril antigen will be performed by somatic cell hybridization techniques using ME-180 cells which will be fused with polyethylene glycol with mouse epidermal cells. The anchoring fibril antigen has reacted with many samples of human skin but is non-reactive with the non-blistered skin of patients with dystrophic epidermolysis bullosa, a disease in which anchoring fibrils are absent as shown by electron microscopy. Attempts to use the anchoring fibril antibody to follow therapy of epidermolysis bullosa will be made both by biopsies of patients and by following the skin biopsies of the epidermolysis bullosa skin transplanted to the nude mouse. Success with these techniques encourages us to use these techniques to form antibodies which will allow dissection of the heterogeneous population of epidermal basal cells and also which distinguishes basal from non-basal cells.

抗表皮和基底膜区抗原的单克隆抗体， 已经形成，新的抗体将通过融合来自 用小鼠骨髓瘤细胞系P3/NS 1免疫小鼠。 免疫的小鼠 与人宫颈癌细胞系（ME-180）形成抗体， 到人类的锚定纤维。 人皮肤中的抗原和ME-180将被 用其分子量、等电点、 肽和溴化氰图谱及氨基酸组成。 的细胞 通过生物合成研究确定皮肤中抗原的来源 和真皮/表皮重组。 的形成 锚定原纤维及其抗原将在 伤口愈合和在表皮细胞附着到胶原包被的 组织培养皿。 锚定纤维抗原的基因定位将 通过使用ME-180细胞的体细胞杂交技术进行 将其与聚乙二醇和小鼠表皮细胞融合。 锚定原纤维抗原与许多人类皮肤样本发生了反应 但与营养不良患者的非红斑皮肤无反应性 大疱性表皮病，一种疾病，其中锚定纤维缺乏， 电子显微镜显示。 尝试使用锚定原纤维 随后治疗大疱性表皮炎的抗体将通过以下两种方法制备： 通过对患者的皮肤活检， 移植至裸鼠的大疱性表皮。 成功与 这些技术鼓励我们使用这些技术来形成抗体 这将允许解剖表皮的异质群体， 基底细胞，也区分基底细胞和非基底细胞。