OPTICAL AND ELECTRICAL STUDIES IN SINGLE MUSCLE FIBERS

单肌纤维的光电研究

• 批准号: 3155281
• 负责人: Julio L Vergara
• 金额: $ 11.36万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3155281
• 关键词: electrophysiology; light emission; membrane potentials; muscle contraction; myocardium; myofibrils; photochemistry; sarcoplasmic reticulum; striated muscles

The research project proposed is aimed at the description, characterization and correlation of events involved in excitation-contraction coupling in twitch muscle fibers. Experiments are proposed for studying events occurring in tubular system membranes, triad and sacroplamsic reticulum membranes in addition to calcium concentration changes in thh sarcoplasm. The membrane potential changes of the tubular system and the sarcoplasmic reticulum will be monitored by voltage-sensing-dyes (fluorescence and absorption). Triadic function will be studied in correlation to charge movements. Calcium concentration changes will be monitored by calcium-sensing-dyes. Modifiers of excitation-contraction coupling will be tested to assess the role of each of the above processes in the physiology of muscle contractions. We will use a cut fiber preparation and double vaseline gap voltage clamp technique allowing modification of the internal medium of the muscle fiber, control of the surface membrane potential and measurement of optical signals. Our experiments are expected to provide new information on 1) the steps involved in excitation-contraction coupling, and 2) the links between consecutive steps. These studies are expected to be of importance in the physiology of striated muscle, including heart muscle.

该研究项目的目的是描述，表征和 抽搐中兴奋-收缩偶联事件的相关性 肌肉纤维 提出了研究管状系统中事件的实验方法 膜、三联体和骶浆网膜 肌浆中的浓度变化。 膜电位的变化 肾小管系统和肌浆网将被监测， 电压敏感染料（荧光和吸收）。 三元函数将是 与电荷运动的相关性 钙浓度的变化将 通过钙敏感染料进行监测。 兴奋-收缩调节剂 将对耦合进行测试，以评估上述每个过程在 肌肉收缩的生理学 我们将使用切割纤维制备， 双凡士林间隙电压钳技术，允许修改的 肌纤维的内部介质，表面膜电位的控制 和光信号的测量。 我们的实验有望在以下方面提供新的信息：1）所涉及的步骤 在兴奋-收缩偶联中，以及2）连续步骤之间的联系。 这些研究对横纹肌的生理学研究具有重要意义 肌肉，包括心脏肌肉。