COLLAGEN BIOSYNTHESIS IN HUMAN SKIN FIBROBLASTS

人类皮肤成纤维细胞中的胶原蛋白生物合成

• 批准号: 3155632
• 负责人: SHELDON R PINNELL
• 金额: $ 14.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-04-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3155632
• 关键词: ascorbate; cell adhesion; cell differentiation; connective tissue disorder; connective tissue metabolism; crosslink; fibroblasts; gel electrophoresis; human subject; human tissue; messenger RNA; osteoporosis; proline; skin; tissue /cell culture; wound healing

Collagen is the major structural protein of the body and recent studies have emphasized its role in other biological processes including differentiation and cell adhesion. Connective tissue abnormalities which may be related to biological properties of collagen occur throughout medicine. These include fibrotic conditions, osteoporosis, wound healing, developmental defects, and aging. Human skin fibroblasts in culture devote a considerable portion of their biosynthetic capacities to collagen production and provide a convenient means for studying the regulation of its synthesis. Collagen synthesis including type-specific synthesis and levels of modifying enzymes (lysyl hydroxylase and prolyl hydroxylase) will be studied. An attempt will be made to understand the role and level of regulatory influences including hormonal and nutritional on collagen production in these cells.

胶原蛋白是人体的主要结构蛋白， 已经强调了它在其他生物过程中的作用， 分化和细胞粘附。 结缔组织异常， 可能与胶原蛋白的生物学特性有关， 药 这些包括纤维化病症、骨质疏松症、伤口愈合， 发育缺陷和衰老。 人皮肤成纤维细胞的培养 相当大一部分的胶原蛋白生物合成能力 生产和提供了一个方便的手段，研究调控 它的合成。 胶原蛋白合成，包括类型特异性合成和 修饰酶（赖氨酰羟化酶和脯氨酰羟化酶）的水平将 被研究。 将试图了解的作用和水平， 调节影响，包括激素和营养对胶原蛋白 在这些细胞中生产。