COLLAGEN BIOSYNTHESIS IN HUMAN SKIN FIBROBLASTS

人类皮肤成纤维细胞中的胶原蛋白生物合成

• 批准号: 3155636
• 负责人: SHELDON R PINNELL
• 金额: $ 14.23万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-04-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3155636
• 关键词: aging; ascorbate; cell adhesion; cell differentiation; collagen; connective tissue disorder; connective tissue metabolism; enzyme linked immunosorbent assay; fibroblasts; genetic library; genetic transcription; glycosylation; human subject; human tissue; laboratory rabbit; messenger RNA; nucleic acid sequence; proline; protein biosynthesis; skin; tissue /cell culture; transforming growth factors; wound healing

Collagen is the major structural protein in the body and one that plays an important role in such diverse biological functions as differentiation, remodeling, wound repair, and aging. Human skin fibroblasts in culture devote a large portion of their protein synthetic capacity to collagen, thus providing a convenient system for studying regulation of collagen synthesis. Our ongoing studies are concerned with regulation of the expression of collagen genes as well as the collagen-specific enzymes prolyl hydroxylase and lysyl hydroxylase, especially with respect to ascorbic acid, an inducer of collagen synthesis, and minoxidil, a drug that we have recently found to specifically inhibit the synthesis of lysyl hydroxylase. We propose studies designed to understand the mechanism of these effects at the molecular level.

胶原蛋白是人体的主要结构蛋白， 在诸如分化， 重塑、伤口修复和老化。 培养的人皮肤成纤维细胞 将其蛋白质合成能力的大部分用于胶原蛋白， 从而为研究胶原蛋白的调节提供了方便的系统， 合成. 我们正在进行的研究关注的是 胶原基因以及胶原特异性酶的表达 脯氨酰羟化酶和赖氨酰羟化酶，特别是关于 抗坏血酸，一种胶原合成的诱导剂，和米诺地尔，一种 我们最近发现可以特异性抑制赖氨酰的合成 羟化酶 我们提出的研究旨在了解的机制， 这些影响在分子水平上。