COLLAGEN BIOSYNTHESIS IN HUMAN SKIN FIBROBLASTS

胶原蛋白是人体内的主要结构蛋白，也是一种发挥 在分化等不同的生物学功能中发挥重要作用， 重塑、伤口修复和老化。体外培养的人皮肤成纤维细胞 将它们蛋白质合成能力的很大一部分用于胶原蛋白， 从而为研究胶原蛋白的调节提供了一个方便的系统 综合。我们正在进行的研究涉及对 胶原蛋白基因及其特异性酶的表达 脯氨酸羟基酶和赖氨酸羟基酶，尤指 抗坏血酸，胶原合成的诱导剂，以及米诺地尔，一种可以 我们最近发现能特异性地抑制赖氨酸的合成 羟基酶。我们建议进行研究，以了解 这些在分子水平上的影响。

Collagen is the major structural protein in the body and one that plays an important role in such diverse biological functions as differentiation, remodeling, wound repair, and aging. Human skin fibroblasts in culture devote a large portion of their protein synthetic capacity to collagen, thus providing a convenient system for studying regulation of collagen synthesis. Our ongoing studies are concerned with regulation of the expression of collagen genes as well as the collagen-specific enzymes prolyl hydroxylase and lysyl hydroxylase, especially with respect to ascorbic acid, an inducer of collagen synthesis, and minoxidil, a drug that we have recently found to specifically inhibit the synthesis of lysyl hydroxylase. We propose studies designed to understand the mechanism of these effects at the molecular level.