COLLAGEN BIOSYNTHESIS IN HUMAN SKIN FIBROBLASTS

胶原蛋白是体内的主要结构蛋白，具有重要的作用 在分化等多种生物功能中发挥着重要作用， 重塑、伤口修复和衰老。 培养中的人皮肤成纤维细胞 将大部分蛋白质合成能力投入到胶原蛋白中， 从而为研究胶原蛋白的调节提供了一个方便的系统 合成。 我们正在进行的研究涉及监管 胶原蛋白基因以及胶原蛋白特异性酶的表达 脯氨酰羟化酶和赖氨酰羟化酶，特别是关于 抗坏血酸（一种胶原蛋白合成诱导剂）和米诺地尔（一种药物） 我们最近发现可以特异性抑制赖氨酰的合成 羟化酶。 我们提出旨在了解其机制的研究 这些效应是在分子水平上产生的。

Collagen is the major structural protein in the body and one that plays an important role in such diverse biological functions as differentiation, remodeling, wound repair, and aging. Human skin fibroblasts in culture devote a large portion of their protein synthetic capacity to collagen, thus providing a convenient system for studying regulation of collagen synthesis. Our ongoing studies are concerned with regulation of the expression of collagen genes as well as the collagen-specific enzymes prolyl hydroxylase and lysyl hydroxylase, especially with respect to ascorbic acid, an inducer of collagen synthesis, and minoxidil, a drug that we have recently found to specifically inhibit the synthesis of lysyl hydroxylase. We propose studies designed to understand the mechanism of these effects at the molecular level.