PHAGOCYTE CYTOSKELETON-COMPLEMENT RECEPTOR INTERACTIONS

白细胞补体(C)系统由八种类型的膜C组成 由白细胞合成的各种C组分的受体和血浆C 与白细胞相互作用的成分。这个项目的目标是 以确定白细胞C系统的功能。具体目标 主要研究内容如下：(1)检测淋巴细胞的体外免疫反应 并确定白细胞C系统是否在识别 另一种途径(AP)激活剂通过B因子裂解 膜C5或因子H(H)与膜H受体(H-R)的结合， CR2对淋巴细胞活化抑制作用的特征 (C3d受体)配体，并确定这是否代表一种 AP的非激活剂的辨别；(2)检查AP的作用 白细胞C系统对巨噬细胞的杀瘤活性及天然 (3)确定血清或效应细胞来源的C3是否 沉积到激活AP的肿瘤细胞上，这是否代表 效应细胞通过C蛋白与肿瘤细胞结合的重要机制 (4)检测白细胞C系统在中性粒细胞中的作用 对酵母多糖和细菌的呼吸爆发反应；和(5)确定 无论是IC3b还是包含在 微生物细胞壁引发呼吸爆发和释放 C受体3(CR3)识别的溶酶体酶。 (CS)

The leukocyte complement (C) system consists of eight types of membrane C receptors, various C components synthesized by leukocytes, and plasma C components that interact with leukocytes. The objective of this project is to characterize the function of the leukocyte C system. The specific aims are as follows: (1) examine the in vitro immune response of lymphocytes and determine if the leukocyte C system has a role in recognition of alternative pathway (AP) activators through either factor B cleavage of membrane C5 or the binding of factor H (H) to membrane H receptors (H-R), characterize the suppression of lymphocyte activation by CR2 (C3d-receptor) ligands and determine if this represents a mechanism for discrimination of nonactivators of the AP; (2) examine the role of the leukocyte C system in the tumoricidal activity of macrophages and natural killer cells; (3) determine whether serum-\or effector cell-derived C3 is deposited onto AP-activating tumor cells and whether this represents an important mechanism for effector cell binding to tumor cells by way of C receptors; (4) examine the role of the leukocyte C system in the neutrophil respiratory burst response to zymosan and bacteria; and (5) determine whether either iC3b or specific carbohydrates contained in the microorganism cell walls trigger a respiratory burst and release of lysosomal enzymes through recognition by C receptor type three (CR3). (CS)