DEFECTS IN NATIVE ANTI-M AVIUM IMMUNITY IN AIDS

艾滋病中天然抗 M 病毒免疫缺陷

• 批准号: 3144708
• 负责人: ALFRED J CROWLE
• 金额: $ 13.87万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3144708
• 关键词: AIDS; HIV infections; Mycobacterium avium; antiserum; colony stimulating factor; disease /disorder model; growth inhibitors; immunity; immunologic skin test; monocyte; phagocytes; secondary infection; virus cytopathogenic effect

Severe infection with Mycobacterium avium is an undesirable, so-far incurable, complication of HIV infection in up to 50% of AIDS patients. This project's objective is to learn why AIDS patients are extraordinarily susceptible to M. avium. The knowledge should help prevent and cure this complication, and should also improve understanding of the pathogenesis and immunology of HIV infection. There are two important defects in AIDS native immunity to M. avium - lack of a serum constituent that blocks M. avium growth in cultured human monocytic phagocytes (MP), and unresponsiveness of MP to the blocker. These defects will be systematically studied with the in vitro model of M. avium growth in MP will be studied. The bacteria in the MP require a nutrient made by the MP from a pronutrient constituent of serum. Conversion of pronutrient to nutrient is blocked in normal MP by an inhibitor in normal serum. AIDS serum contains the pronutrient but lacks the inhibitor, and AIDS MP do not respond to the inhibitor. A defined serum substitute will be used. It resembles AIDS serum in containing the pronutrient but lacking the inhibitor. The pronutrient will be identified from among the known constituent(s) of normal serum makes the serum substitute inhibitory. The defect in AIDS MP will be analyzed by studying the responses of MP from subjects in various stages of HIV infection, compared with MP from normal subjects, to M. avium infection in normal and patient serum, an in serum substitute. The experimental model uses cultured human MP infected with virulent M. avium, where changes in concentration of colony-forming units of M. avium in the MP and their medium are measured at 0, 4, and 7 days after infection.

严重感染鸟分枝杆菌是一种不良的，如此野性 在多达50％的艾滋病患者中，无法治愈的HIV感染并发症。 该项目的目标是了解为什么艾滋病患者非常 容易受到雄性大麻菌的影响。 知识应有助于防止和治愈 并发症，还应提高对发病机理和 HIV感染的免疫学。 艾滋病有两个重要的缺陷 天然对雄性大麻菌的免疫力 - 缺乏阻塞M.的血清成分。 培养的人类单核细胞（MP）和 国会议员对阻止者的反应症。 这些缺陷将是 系统地研究了MP的体外体外模型 将被研究。 MP中的细菌需要MP制造的营养 来自血清的前营养成分。 proto剂量转化为 正常血清中抑制剂在正常MP中阻塞营养素。 艾滋病 血清中含有前营养素，但缺乏抑制剂，艾滋病MP不 响应抑制剂。 将使用定义的血清替代品。 它 类似于有助于血清的含有前锋，但缺乏 抑制剂。 可从已知的 正常血清的组成使血清替代抑制作用。 这 AIDS MP缺陷将通过研究MP的响应来分析 与来自HIV感染的各个阶段的受试者相比 正常受试者，正常和患者血清中的鸟杆菌感染， 血清替代品。 实验模型使用培养的人MP感染 与雄性雄性分枝杆菌一起，菌落形成的浓度变化 MP及其培养基中的鸟藻单位在0、4和7中测量 感染后几天。