PHYSIOLOGY OF HYPOTHALAMIC NEUROHORMONES

我们建议继续我们目前关于下丘脑生理学的项目 调节脑下垂体激素分泌的神经激素。重点将是 放在描绘生长激素释放激素(GHRH)的释放上， 催乳素释放激素(PRH)和皮质醇释放激素(CRH) 进入大鼠低甲状腺素门静脉血并测定其特性 下丘脑荷尔蒙。将测量脑下垂体激素的释放活性 使用多通道灌流系统，然后测量垂体腺 灌流中的荷尔蒙由适当的RIA产生。如果这样的活动 都存在于大鼠脑垂体门静脉血中，我们将评估这种激素 不同电针刺激后门脉血的释放活性 大脑中已知的刺激各自脑下垂体释放的区域 激素和脑室内注射各种神经递质。这将是 作为对心脏功能的生理重要性的初步评估。 观察到激素释放活动，并将提供丰富的释放激素 门脉血。浓缩的门静脉血随后将被汇集、提取和 所检测活性物质的理化特性。并行的 研究表明，从大豆中提取的活性物质的物理化学性质 大鼠、家畜和大鼠脑垂体的下丘脑提取物 门静脉血将被比对。如果任何部分被发现含有重要的 GHRH、PRH或CRH活性，将努力分离这些激素。 大鼠和家养动物的胰腺和肠道组织提取物也将 接受这些释放活动的检查。如果有任何下丘脑外部分 被发现具有这些活性，将努力分离出这些 物质及其与活性物质的物理化学性质的比较 在大鼠脑垂体门静脉血和下丘脑提取物中发现的物质。

We propose to continue our current project on the physiology of the hypothalamic neurohormones which regulate secretion of pituitary hormones. Emphasis will be placed on delineating the release of growth hormone releasing hormone (GHRH), prolactin releasing hormone (PRH), and corticotronin releasing hormone (CRH) into the rat hypohyseal portal blood and determining characteristics of these hypothalamic hormones. Pituitary hormone releasing activity will be measured using a multichannel perifusion system followed by measurement of the pituitary hormones in the perifusion output by the appropriate RIA. If such activities are present in the rat hypophyseal portal blood, we will assess the hormone releasing activity of portal blood following electrical stimulation of various regions of the brain known to stimulate the release of the respective pituitary hormone and intraventricular injection of various neurotransmitters. This will serve as a preliminary assessment of the physiological importance of the observed hormone releasing activity and will provide releasing hormone-enriched portal blood. The enriched portal blood will then be pooled, extracted and the physicochemical characteristics of the active substances examined. In parallel studies, the physico-chemical properties of active substances obtained from hypothalamic extracts of rats and domestic animals and from rat hypophyseal portal blood will be compared. If any fraction is found to contain significant GHRH, PRH, or CRH activity, an effort will be made to isolate these hormones. Extracts of pancreatic and gut tissue from rats and domestic animals will also be examined for these releasing activities. If any extrahypothalamic fraction is found to possess these activities an effort will be made to isolate these substances, and their physico-chemical properties compared to those of active substances found in rat hypophyseal portal blood and hypothalamic extracts.