PHYSIOLOGY OF HYPOTHALAMIC NEUROHORMONES

分离和鉴定了TRH、LHRH、生长抑素、CFR和GHRF。 他们的合成制剂已经上市。这些下丘脑 摘要多肽被认为是一种生理性促垂体激素。 它们调节各自脑下垂体中的靶细胞。 然而，我们最近的实验表明，生长激素的释放 不仅通过生长抑素和GHRF(大鼠的情况下是大鼠的GHRF43)，而且 还有其他未知因素(S)。此外，我们还分离出了一种多肽 羊脑提取物促进生长激素释放的体外实验及鉴定 其部分氨基酸序列为HSDGIFTDSYKRYNKEMAK。另一方面 另一方面，催乳素释放因子(PRF)还没有被分离出来，尽管VIP和 PH127已被提议作为PRF。与悲观的观点相关的争论 除LHRH外，FSHRH还没有得到解决。这些问题导致了 美国决定重新探索可能的新型促垂体多肽 通过标记非特异性检测的活性多肽来定位下丘脑 环核苷酸刺激测定大鼠下丘脑活动 与已知的下丘脑因素进行比较。如果存在新的多肽， 它们的具体促垂体活性将被测定，并最终 它们的分离、表征、合成和生理研究 意义。此外，我们还展示了CRF和GHRF在 直觉。将对这些物质进行临床和免疫学检查 与下丘脑对应的特征相比较，如果一个 如果存在足够的数量，它们将被分离、表征和 它们的生理意义将被确定。最后， 下丘脑神经元合成和合成神经递质的调节机制 释放下丘脑激素，特别是CRF和GHRF。 这些机制将用生理学和 免疫组织化学方法。免疫组织化学的工作将是 在电子显微镜水平上采用双重染色方法。

TRH, LHRH, somatostatin, CFR and GHRF were isolated and characterized, and their synthetic preparations have become available. These hypothalamic peptides were considered to be the physiological hypophysiotropic hormones which regulate their respective target cells in the pituitary gland. However, our recent experiments have suggested that GH release is resulated not only by somatostatin and GHRF (rat GHRF43 in the case of rats), but also other unknown factor(s). Furthermore, we have isolated a peptide from ovine brain extracts which stimulates GH release in vitro and elucidated its partial amino acid sequence as HSDGIFTDSYKRYNKEMAK... On the other hand, PRL releasing factor (PRF) has not been isolated, though VIP and PH127 have been proposed as a PRF. Arguments relative to the pesence of FSHRH in addition to LHRH have not been settled. These problems have led us to decide to reexplore possible novel hypophysiotropic peptides in the hypothalamus by mapping active peptides as tested by non-specific hypothalamic activity as determined by cyclic nucleotide stimulation in comparison with known hypothalamic factors. If novel peptides are present, their specific hypophysiotropic activity will be determined and eventually isolated characterized, synthetized and studied for their physiological significance. In addition, we demonstrated CRF and GHRF activity in the gut. These substances will be examined for chamical and immunological characteristics in comparison with hypothalamic counterparts, and if a sufficient amount is present, they will be isolated, characterized and their physiological significance will be determined. Finally, the regulatory mechanism of the hypothalamic neurons which synthetize and release hypothalamic hormones, with a special emphasis on CRF and GHRF. These mechanisms will be investigated using physiological and immunohistochemical methods. The immunohistochemical work will be conducted using double staining method at electron nicroscopic level.