PHYSIOLOGY OF HYPOTHALAMIC NEUROHORMONES

TRH、LHRH、生长抑素、CFR和GHRF被分离和表征， 它们的合成制剂已经可以获得。 这些下丘脑 肽被认为是生理性促垂体激素 它们调节脑垂体中各自的靶细胞。 然而，我们最近的实验表明，GH的释放是可逆的， 不仅通过生长抑素和GHRF（大鼠GHRF 43）， 还有其他未知因素。 此外，我们还分离了一种肽， 羊脑提取物刺激GH释放的体外研究 其部分氨基酸序列为HSDGIFTDSYKRYNKEMAK。 另 另一方面，PRL释放因子（PRF）尚未分离，尽管VIP和 PH127已被提议作为PRF。 与下列问题有关的论点： 除了LHRH之外，FSHRH也没有得到解决。 这些问题导致 我们决定重新探索可能的新型促垂体肽， 下丘脑通过映射活性肽作为测试的非特异性 通过环核苷酸刺激测定下丘脑活性， 与已知的下丘脑因素比较。 如果存在新的肽， 它们的特定促垂体活性将被确定， 分离、表征、合成并研究其生理活性 意义 此外，我们证明了CRF和GHRF活性在 直觉 将对这些物质进行查米卡尔和免疫学检查 与下丘脑对应物相比， 如果存在足够量，则将它们分离、表征并 将确定它们的生理意义。 最后 下丘脑神经元的调节机制， 释放下丘脑激素，特别强调CRF和GHRF。 这些机制将使用生理和 免疫组织化学方法。 免疫组织化学工作将是 在电镜水平上使用双重染色法进行。

TRH, LHRH, somatostatin, CFR and GHRF were isolated and characterized, and their synthetic preparations have become available. These hypothalamic peptides were considered to be the physiological hypophysiotropic hormones which regulate their respective target cells in the pituitary gland. However, our recent experiments have suggested that GH release is resulated not only by somatostatin and GHRF (rat GHRF43 in the case of rats), but also other unknown factor(s). Furthermore, we have isolated a peptide from ovine brain extracts which stimulates GH release in vitro and elucidated its partial amino acid sequence as HSDGIFTDSYKRYNKEMAK... On the other hand, PRL releasing factor (PRF) has not been isolated, though VIP and PH127 have been proposed as a PRF. Arguments relative to the pesence of FSHRH in addition to LHRH have not been settled. These problems have led us to decide to reexplore possible novel hypophysiotropic peptides in the hypothalamus by mapping active peptides as tested by non-specific hypothalamic activity as determined by cyclic nucleotide stimulation in comparison with known hypothalamic factors. If novel peptides are present, their specific hypophysiotropic activity will be determined and eventually isolated characterized, synthetized and studied for their physiological significance. In addition, we demonstrated CRF and GHRF activity in the gut. These substances will be examined for chamical and immunological characteristics in comparison with hypothalamic counterparts, and if a sufficient amount is present, they will be isolated, characterized and their physiological significance will be determined. Finally, the regulatory mechanism of the hypothalamic neurons which synthetize and release hypothalamic hormones, with a special emphasis on CRF and GHRF. These mechanisms will be investigated using physiological and immunohistochemical methods. The immunohistochemical work will be conducted using double staining method at electron nicroscopic level.