Exploiting plant synthetic biology for the production of glycoproteins in plant chloroplasts.

疫苗接种在减轻传染病的健康负担方面发挥了重要作用。它提供了一种有效的途径，通过这种途径，通常可以有效地根除年轻人的严重感染。预防感染最有效的疫苗是结合疫苗。它们是通过将病原体的多糖与载体蛋白化学连接而成的。目前生产结合疫苗的方法的一个主要缺点是生产成本和与大量病原体生长有关的危险。这项工作将通过直接在植物叶绿体中合成结合疫苗来解决这些问题。叶绿体是植物进行光合作用的细胞器。将结合疫苗生产定位在叶绿体内部将有助于纯化并允许生产大量材料。在未来，这种方法可能允许从可食用植物中纯化和制造用于口服免疫的疫苗。

Vaccination has played a major role in reducing the health burden of infectious diseases. It offers an effective route by which serious infections, usually of the young, can be effectively eradicated. The most effective vaccines to prevent infections are conjugate vaccines. These are made by chemically joining a polysaccharide from the pathogen to a carrier protein. A major drawback of the current methodology for the generation of conjugate vaccine is the cost of production and the hazards associated with growing large amounts of pathogen. This proposed work will address these problems by synthesising conjugate vaccines directly in plant chloroplasts. Chloroplasts are plant organelles that carry out photosynthesis. Localising conjugate vaccine production inside the chloroplast will facilitate purification and permit large amounts of material to be produced. In the future this approach may allow the purification and manufacture of vaccines from edible plants for oral immunisation.