Exploiting plant synthetic biology for the production of glycoproteins in plant chloroplasts.

利用植物合成生物学在植物叶绿体中生产糖蛋白。

• 批准号: BB/J019070/1
• 负责人: Ian Roberts
• 金额: $ 15.27万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ019070%2F1
• 关键词: Exploiting; plant; synthetic; biology; production

Vaccination has played a major role in reducing the health burden of infectious diseases. It offers an effective route by which serious infections, usually of the young, can be effectively eradicated. The most effective vaccines to prevent infections are conjugate vaccines. These are made by chemically joining a polysaccharide from the pathogen to a carrier protein. A major drawback of the current methodology for the generation of conjugate vaccine is the cost of production and the hazards associated with growing large amounts of pathogen. This proposed work will address these problems by synthesising conjugate vaccines directly in plant chloroplasts. Chloroplasts are plant organelles that carry out photosynthesis. Localising conjugate vaccine production inside the chloroplast will facilitate purification and permit large amounts of material to be produced. In the future this approach may allow the purification and manufacture of vaccines from edible plants for oral immunisation.

疫苗接种在减少传染病的健康负担方面发挥了重要作用。它提供了一个有效的途径，通过它可以有效地根除严重的感染，通常是年轻人的感染。预防感染最有效的疫苗是结合疫苗。这些是通过化学方法将病原体的多糖与载体蛋白结合而成的。目前用于产生缀合物疫苗的方法的主要缺点是生产成本和与大量病原体生长相关的危害。这项拟议的工作将通过直接在植物叶绿体中合成缀合疫苗来解决这些问题。叶绿体是进行光合作用的植物细胞器。将结合疫苗生产定位在叶绿体内将促进纯化并允许产生大量材料。在未来，这种方法可能允许从可食用植物中纯化和制造用于口服免疫的疫苗。

项目成果

Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines.

• DOI: 10.1371/journal.pone.0088144
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Smith CM;Fry SC;Gough KC;Patel AJ;Glenn S;Goldrick M;Roberts IS;Whitelam GC;Andrew PW
• 通讯作者: Andrew PW